Manilkara zapota L. extract topical ointment application to skin wounds in rats speeds up the healing process.

Autor: Alsareii SA; Department of Surgery, College of Medicine, Najran University, Najran, Saudi Arabia., Alzerwi NAN; Department of Surgery, College of Medicine, Majmaah University, Ministry of Education, Al-Majmaah, Saudi Arabia., Alasmari MY; Department of Surgery, College of Medicine, Najran University, Najran, Saudi Arabia., Alamri AM; Department of Surgery, College of Medicine, Najran University, Najran, Saudi Arabia., Mahnashi MH; Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran, Saudi Arabia., Shaikh IA; Department of Pharmacology, College of Pharmacy, Najran University, Najran, Saudi Arabia., Savant C; Department of Pharmacology, SET's College of Pharmacy, Dharwad, Karnataka, India., Kulkarni PV; Department of Pharmacology, SET's College of Pharmacy, Dharwad, Karnataka, India., Shettar AK; Division of Preclinical Research and Drug Development, Cytxon Biosolutions Pvt. Ltd., Hubli, Karnataka, India., Hoskeri JH; Department of Bioinformatics and Biotechnology, Karnataka State Akkamahadevi Women's University, Vijayapura, Karnataka, India., Kumbar V; Dr. Prabhakar Kore Basic Science Research Centre, KLE Academy of Higher Education and Research (KLE University), Belagavi, Karnataka, India.
Jazyk: angličtina
Zdroj: Frontiers in pharmacology [Front Pharmacol] 2023 Jun 29; Vol. 14, pp. 1206438. Date of Electronic Publication: 2023 Jun 29 (Print Publication: 2023).
DOI: 10.3389/fphar.2023.1206438
Abstrakt: Poor circulation, unresolved inflammation, neuropathy, and infection make wound care difficult. Manilkara zapota ( M. zapota ) antibacterial and antioxidant properties may help speed up the healing process. The present investigation aimed to evaluate the wound healing activity of M. zapota bark ethanolic extract (MZE) by employing in-vitro migration scratch assay and in-vivo animal models. Wistar albino rats were used for the in-vivo wound healing models. No treatment was given to Group I; Group II received povidone-iodine (5% W/W); Group III received MZE (5% W/W); and Group IV received MZE (10% W/W). Linear incision models and excision wound models were used to induce injury. The ointments were applied immediately to the wounds after causing the injury. The percentage of wound contraction, the length of the epithelization period, and the wound's tensile strength were all calculated. The scratch assay assessed the test drug's potential for wound healing in-vitro . H 2 O 2 and DPPH scavenging assays were used to measure antioxidant activity. A p < 0.05 was used to define statistical significance. On days 4, 8, 12, 16, and 20, the wound contraction potential of animals treated with MZE ointment was significantly higher ( p < 0.001) than that of the control group. On day 20, the proportion of wound contraction in MZE-treated animals was 99.88%, compared to 83.86% in untreated animals. The test group had a significantly ( p < 0.01) faster time to full epithelization than the control group. In the incision model, the control group had considerably lower mechanical strength ( p < 0.001) than animals treated with MZE. In addition, MZE caused a significant increase ( p < 0.001) in total protein and hydroxyproline levels. In the scratch experiment, test drug-treated cells showed a higher rate of cell migration than untreated cells. Furthermore, animals treated with MZE showed increased levels of epithelial tissue, collagen proliferation, and keratinization. To summarize, the current study found that M. zapota improved wound healing activity both in vitro and in vivo , as evidenced by the study results. M. zapota extract has significant wound-healing potential and could be a viable source of wound-healing nutraceuticals.
Competing Interests: AS was employed by the company Cytxon Biosolutions Pvt. Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Alsareii, Alzerwi, Alasmari, Alamri, Mahnashi, Shaikh, Savant, Kulkarni, Shettar, Hoskeri and Kumbar.)
Databáze: MEDLINE
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