Effect of pulsed laser parameters on photoacoustic flow cytometry efficiency in vitro and in vivo.

Autor: Grishin OV; Science Medical Center, Saratov State University, Saratov, Russia., Shushunova NA; Science Medical Center, Saratov State University, Saratov, Russia., Bratashov DN; Science Medical Center, Saratov State University, Saratov, Russia., Prikhozhdenko ES; Science Medical Center, Saratov State University, Saratov, Russia., Verkhovskii RA; Science Medical Center, Saratov State University, Saratov, Russia., Kozlova AA; Science Medical Center, Saratov State University, Saratov, Russia., Abdurashitov AS; A.V. Zelmann Center for Neurobiology and Brain Rehabilitation, Skolkovo Institute of Science and Technology, Moscow, Russia., Sindeeva OA; A.V. Zelmann Center for Neurobiology and Brain Rehabilitation, Skolkovo Institute of Science and Technology, Moscow, Russia., Karavaev AS; Science Medical Center, Saratov State University, Saratov, Russia.; Laboratory of Nonlinear Dynamics Modeling, Saratov Branch of the Institute of Radio-Engineering and Electronics of Russian Academy of Sciences, Saratov, Russia.; Department of Innovative Cardiological Information Technology, Institute of Cardiological Research, Saratov State Medical University, Saratov, Russia., Kulminskiy DD; Laboratory of Nonlinear Dynamics Modeling, Saratov Branch of the Institute of Radio-Engineering and Electronics of Russian Academy of Sciences, Saratov, Russia.; Scientific Center for Information Technologies and Artificial Intelligence, Sirius University of Science and Technology, Sochi, Russia., Shashkov EV; Pico-Femtoseconds Laser Laboratory, Photoelectronics Department, Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia., Inozemtseva OA; Science Medical Center, Saratov State University, Saratov, Russia., Tuchin VV; Science Medical Center, Saratov State University, Saratov, Russia.; Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk, Russia.; Institute of Precision Mechanics and Control, FRC 'Saratov Scientific Centre of the Russian Academy of Sciences', Saratov, Russia.; Bach Institute of Biochemistry, FRC 'Fundamentals of Biotechnology of the Russian Academy of Sciences', Moscow, Russia.
Jazyk: angličtina
Zdroj: Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2023 Nov; Vol. 103 (11), pp. 868-880. Date of Electronic Publication: 2023 Jul 29.
DOI: 10.1002/cyto.a.24778
Abstrakt: Photoacoustic flow cytometry is one of the most effective approaches to detect "alien" objects in the bloodstream, including circulating tumor cells, blood clots, parasites, and emboli. However, the possibility of detecting high-amplitude signals from these objects against the background of blood depends on the parameters of the laser pulse. So, the dependencies of photoacoustic signals amplitude and number on laser pulse energy (5-150 μJ), pulse length (1, 2, 5 ns), and pulse repetition rate (2, 5, 10 kHz) for the melanoma cells were investigated. First, the PA responses of a melanoma cell suspension in vitro were measured to directly assess the efficiency of converting laser light into an acoustic signal. After it, the same dependence with the developed murine model based on constant rate melanoma cell injection into the animal blood flow was tested. Both in vivo and in vitro experiments show that signal generation efficiency increases with laser pulse energy above 15 μJ. Shorter pulses, especially 1 ns, provide more efficient signal generation as well as higher pulse rates. A higher pulse rate also provides more efficient signal generation, but also leads to overheating of the skin. The results show the limits where the photoacoustic flow cytometry system can be effectively used for the detection of circulating tumor cells in undiluted blood both for in vitro experiments and for in vivo murine models.
(© 2023 International Society for Advancement of Cytometry.)
Databáze: MEDLINE
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