Refinement of Rf1-gene localization and development of the new molecular markers for fertility restoration in sunflower.

Autor: Sivolapova AB; Russian Potato Research Center, 23 Lorkh Str., Kraskovo, Moscow Region, Lyubertsy District, Lyubertsy, 140051, Russia. asivolapova@yahoo.com., Polivanova OB; Russian Potato Research Center, 23 Lorkh Str., Kraskovo, Moscow Region, Lyubertsy District, Lyubertsy, 140051, Russia.; Russian State Agrarian University Moscow Timiryazev Agricultural Academy, 49 Timiryazevskaya Street, Moscow, 127550, Russia., Goryunov DV; Russian Potato Research Center, 23 Lorkh Str., Kraskovo, Moscow Region, Lyubertsy District, Lyubertsy, 140051, Russia.; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1 Leninskie Gory Str., bld. 40, Moscow, 119992, Russia., Chebanova YV; FSBSI Federal scientific center 'V.S. Pustovoit All-Russian Research Institute of Oil crops', 17 imeny Filatova Str, Krasnodar, 350038, Russia., Fedorova AV; Tsitsin Main Botanical Garden Russian Academy of Science, 4 Botanicheskaya Str, Moscow, 127276, Russia., Sotnikova EA; National Medical Research Center for Therapy and Preventive Medicine, 10 Petroverigsky Per., bld. 3, Moscow, 101000, Russia., Karabitsina YI; The N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources, 42, 44, Bolshaya Morskaya, Str., Saint Petersburg, 190000, Russia., Benko NI; Breeding and seed production company 'Agroplazma', 71 Krasnykh Partizan Str, Krasnodar, 350012, Russia., Mukhina ZM; All-Russia Rice Research Institute, 3 Belozernyy poselok, Krasnodar, 350921, Russia., Anisimova IN; The N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources, 42, 44, Bolshaya Morskaya, Str., Saint Petersburg, 190000, Russia., Demurin YN; FSBSI Federal scientific center 'V.S. Pustovoit All-Russian Research Institute of Oil crops', 17 imeny Filatova Str, Krasnodar, 350038, Russia., Goryunova SV; Russian Potato Research Center, 23 Lorkh Str., Kraskovo, Moscow Region, Lyubertsy District, Lyubertsy, 140051, Russia.; Institute of General Genetics Russian Academy of Science, 3 Gubkina Str, Moscow, 119333, Russia.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2023 Sep; Vol. 50 (9), pp. 7919-7926. Date of Electronic Publication: 2023 Jul 15.
DOI: 10.1007/s11033-023-08646-4
Abstrakt: Background: Ability to restore male fertility is important trait for sunflower breeding. The most commonly used fertility restoration gene in the production of sunflower hybrids is Rf1. The localization of Rf1 on the linkage group 13 has been previously shown, however, its exact position, its sequence and molecular mechanism for fertility restoration remain unknown. Therefore, several markers linked to Rf1 gene, commonly used for MAS, don't always allow to identify the genotype of plants. For this reason, the search for new markers and precise localization of the Rf1 gene is an urgent task.
Methods and Results: Based on previously identified single nucleotide polymorphisms (SNPs) at LG13, significantly associated with the ability to restore male fertility, two markers have been developed that have performed well after careful evaluation. These markers, together with other Rf1 markers, were applied for genotyping 72 diversity panel accessions and 291 individuals of F2 segregating population, obtained from crossing the cytoplasmic male sterility (CMS) AHO33 and restorer RT085HO lines. The analysis revealed no recombinants between Rf1 gene and SRF833 marker, the distance between Rf1 and SRF122 marker was 1.0 cM.
Conclusions: Data obtained made it possible to specify the localization of the Rf1 gene and reduce the list of candidate genes to the 3 closely linked PPR-genes spanning a total of 59 Kb. However, it cannot be ruled out that analysis of the candidate region in the genome of fertility restorer lines can reveal new candidate genes in this locus that are absent in the cytoplasmic male sterility maintainer reference sequence.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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