Establishment and validation of a calibration curve for dicentric chromosome induced by 6MV X-ray.

Autor:	Kosarnia M; Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9138813944, Iran., Bahreyni-Toossi MT; Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad 9138813944, Iran., Gholamhosseinian H; Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad 9138813944, Iran., Dolat E; Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9138813944, Iran., Fakour-Mollaee P; Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9138813944, Iran., Azimian H; Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad 9138813944, Iran.
Jazyk:	angličtina
Zdroj:	Radiation protection dosimetry [Radiat Prot Dosimetry] 2023 Aug 09; Vol. 199 (13), pp. 1410-1415.
DOI:	10.1093/rpd/ncad200
Abstrakt:	Radiation during radiotherapy and nuclear accidents is currently one of the biggest concerns for the international community. Biological dosimetry examines the amount of damage caused by radiation at the cellular level by quantifying a radiation biomarker. In particular, the dicentric chromosome assay is a biodosimetric technique that can quantify radiation damage by correlating radiation dose exposure with the frequency of dicentric chromosomes in the peripheral lymphocytes extracted from exposed individuals. This study aims to present of the reference dose-response calibration curve for biodosimetry laboratory of Mashhad University of Medical Sciences (north-east of Iran). In all, 40 samples of peripheral blood from four healthy volunteers were irradiated at doses of 0-5 Gray in a customised water phantom using a 6 MV X-rays at dose rate of 2 Gy/min from a linear accelerator. The irradiated samples were cultured and analysed according to the International Atomic Energy Agency Cytogenetic Dosimetry Protocol (2011) with some modifications. Linear-quadratic model curve fitting and further statistical analysis were done using Chromosome Aberration Calculation Software Version 2.0 and Dose Estimate (Version 5.2). The curve equation obtained was ${Y}_{dic}=0.0533{D}^2+0.0231D+0.0001$ and was in the range of other studies. Validation of the calibration curve was done by estimating the dose of blind samples. (© The Author(s) 2023. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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