RT-qPCR-based pool testing for the diagnosis of COVID-19.

Autor: Sato HI; Vaccine Technology Center, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Costa MS; Postgraduate Program in Infectious Diseases and Tropical Medicine, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Takahashi RHC; Mathematics Department, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Lourenço KL; Vaccine Technology Center, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Guimarães NS; Graduate Program in Collective Health, Universidade Federal da Bahia, Salvador, BA, Brazil., Alves CRL; Department of Pediatrics, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Machado EL; Department of Preventive and Social Medicine, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Tupinambás U; Medical Clinic Department, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Fonseca FGD; Vaccine Technology Center, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Teixeira SMR; Vaccine Technology Center, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Jazyk: angličtina
Zdroj: Einstein (Sao Paulo, Brazil) [Einstein (Sao Paulo)] 2023 Jul 10; Vol. 21, pp. eAE0115. Date of Electronic Publication: 2023 Jul 10 (Print Publication: 2023).
DOI: 10.31744/einstein_journal/2023AE0115
Abstrakt: This study proposes a strategy for large-scale testing among a large number of people for the early diagnosis of COVID-19 to elucidate the epidemiological situation. Pool testing involves the analysis of pooled samples. This study aimed to discuss a reverse transcription technique followed by quantitative real-time polymerase chain reaction using pool testing to detect SARS-CoV-2 in nasopharyngeal swab samples. The study proposes an innovative diagnostic strategy that contributes to resource optimization, cost reduction, and improved agility of feedback from results. Pool testing is simultaneously performed on multiple samples to efficiently and cost-effectively detect COVID-19. Pool testing can optimize resource utilization and expand diagnostic access, and is a viable alternative for developing countries with limited access to testing. To optimize resources, the pool size was determined by estimating COVID-19 prevalence in the study population.
Databáze: MEDLINE
Externí odkaz: