Unraveling the chemical profile, antioxidant, enzyme inhibitory, cytotoxic potential of different extracts from Astragalus caraganae.

Autor: Kurt-Celep İ; Faculty of Pharmacy, Department of Pharmacognosy, Ataşehir, Acıbadem Mehmet Ali Aydınlar University, İstanbul, Turkey., Zengin G; Department of Biology, Science Faculty, Selcuk University, Konya, Turkey., Uba AI; Department of Molecular Biology and Genetics, Istanbul AREL University, Istanbul, Turkey., Caprioli G; School of Pharmacy, University of Camerino, Camerino, Italy., Mustafa AM; School of Pharmacy, University of Camerino, Camerino, Italy., Angeloni S; School of Pharmacy, University of Camerino, Camerino, Italy., Cakilcioglu U; Pertek Sakine Genç Vocational School, Munzur University, Pertek, Turkey., Guler O; Pertek Sakine Genç Vocational School, Munzur University, Pertek, Turkey., Kaplan A; Sason Vocational School, Batman University, Batman, Turkey., Sharmeen J; Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Reduit, Mauritius., Mahomoodally MF; Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Reduit, Mauritius.; Department of Pharmacology, Center for Transdisciplinary Research, Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Saveetha University, Chennai, India.; Centre of Excellence for Pharmaceutical Sciences, North-West University, Potchefstroom, South Africa.
Jazyk: angličtina
Zdroj: Archiv der Pharmazie [Arch Pharm (Weinheim)] 2023 Sep; Vol. 356 (9), pp. e2300263. Date of Electronic Publication: 2023 Jul 11.
DOI: 10.1002/ardp.202300263
Abstrakt: Six extracts (water, ethanol, ethanol-water, ethyl acetate, dichloromethane, and n-hexane) of Astragalus caraganae were studied for their biological activities and bioactive contents. Based on high-performance liquid chromatography-mass spectrometry (HPLC-MS), the ethanol-water extract yielded the highest total bioactive content (4242.90 µg g -1 ), followed by the ethanol and water extracts (3721.24 and 3661.37 µg g -1 , respectively), while the least total bioactive content was yielded by the hexane extract, followed by the dichloromethane and ethyl acetate extracts (47.44, 274.68, and 688.89 µg g -1 , respectively). Rutin, p-coumaric, chlorogenic, isoquercitrin, and delphindin-3,5-diglucoside were among the major components. Unlike the dichloromethane extracts, all the other extracts showed radical scavenging ability in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay (8.73-52.11 mg Trolox equivalent [TE]/g), while all extracts displayed scavenging property in the 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging assay (16.18-282.74 mg TE/g). The extracts showed antiacetylcholinesterase (1.27-2.73 mg galantamine equivalent [GALAE]/g), antibutyrylcholinesterase (0.20-5.57 mg GALAE/g) and antityrosinase (9.37-63.56 mg kojic acid equivalent [KAE]/g) effects. The molecular mechanism of the H 2 O 2 -induced oxidative stress pathway was aimed to be elucidated by applying ethanol, ethanol/water and water extracts at 200 µg/mL concentration to human dermal cells (HDFs). A. caraganae in HDF cells had neither a cytotoxic nor genotoxic effect but could have a cytostatic effect in increasing concentrations. The findings have allowed a better insight into the pharmacological potential of the plant, with respect to their chemical entities and bioactive contents, as well as extraction solvents and their polarity.
(© 2023 Deutsche Pharmazeutische Gesellschaft.)
Databáze: MEDLINE
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