Characterization of three Francisella tularensis genomes from Oklahoma, USA.

Autor:	Narayanan S; Oklahoma Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Oklahoma State University, 1950 W Farm Rd, Stillwater, OK 74078, USA., Couger B; Brigham and Women's Hospital, Harvard Medical School, 75 Francis St., Boston, MA 02115, USA., Bates H; Yale School of Nursing, 400 W Campus Dr., Orange, CT 06477, USA., Gupta SK; Oklahoma Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Oklahoma State University, 1950 W Farm Rd, Stillwater, OK 74078, USA., Malayer J; College of Veterinary Medicine, Oklahoma State University, 208 S McFarland St., Stillwater, OK 74078, USA., Ramachandran A; Oklahoma Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Oklahoma State University, 1950 W Farm Rd, Stillwater, OK 74078, USA.
Jazyk:	angličtina
Zdroj:	Access microbiology [Access Microbiol] 2023 Jun 14; Vol. 5 (6), pp. acmi000451. Date of Electronic Publication: 2023 Jun 14 (Print Publication: 2023).
DOI:	10.1099/acmi.0.000451
Abstrakt:	Francisella tularensis , the causative agent for tularaemia, is a Tier 1 select agent, and a pan-species pathogen of global significance due to its zoonotic potential. Consistent genome characterization of the pathogen is essential to identify novel genes, virulence factors, antimicrobial resistance genes, for studying phylogenetics and other features of interest. This study was conducted to understand the genetic variations among genomes of F. tularensis isolated from two felines and one human source. Pan-genome analysis revealed that 97.7 % of genes were part of the core genome. All three F. tularensis isolates were assigned to sequence type A based on single nucleotide polymorphisms (SNPs) in sdhA . Most of the virulence genes were part of the core genome. An antibiotic resistance gene coding for class A beta-lactamase was detected in all three isolates. Phylogenetic analysis showed that these isolates clustered with other isolates reported from Central and South-Central USA. Assessment of large sets of the F. tularensis genome sequences is essential in understanding pathogen dynamics, geographical distribution and potential zoonotic implications. Competing Interests: The authors declare no conflict of interest. (© 2023 The Authors.)
Databáze:	MEDLINE
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