High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217.
Autor: | Li F; Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA., Hou CD; Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA., Lokareddy RK; Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA., Yang R; Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA., Forti F; Dipartimento di Bioscienze, Università degli Studi di Milano, Milan, Italy., Briani F; Dipartimento di Bioscienze, Università degli Studi di Milano, Milan, Italy. federica.briani@unimi.it., Cingolani G; Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA. gino.cingolani@jefferson.edu. |
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Jazyk: | angličtina |
Zdroj: | Nature communications [Nat Commun] 2023 Jul 08; Vol. 14 (1), pp. 4052. Date of Electronic Publication: 2023 Jul 08. |
DOI: | 10.1038/s41467-023-39756-z |
Abstrakt: | E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 Å and 4.5 Å resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4. (© 2023. The Author(s).) |
Databáze: | MEDLINE |
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