The response of human macrophages to 3D printed titanium antibacterial implants does not affect the osteogenic differentiation of hMSCs.

Autor:	Garmendia Urdalleta A; Department of Biomechanical Engineering, Faculty of Mechanical, Maritime and Materials Engineering, TU Delft, Delft, Netherlands.; Department of Oral and Maxillofacial Surgery, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands., Van Poll M; Department of Biomechanical Engineering, Faculty of Mechanical, Maritime and Materials Engineering, TU Delft, Delft, Netherlands.; Department of Oral and Maxillofacial Surgery, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands., Fahy N; Department of Oral and Maxillofacial Surgery, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands.; Department of Orthopaedics, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands.; Department of Applied Science, Technological University of the Shannon: Midlands Midwest, Limerick, Ireland., Witte-Bouma J; Department of Oral and Maxillofacial Surgery, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands., Van Wamel W; Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands., Apachitei I; Department of Biomechanical Engineering, Faculty of Mechanical, Maritime and Materials Engineering, TU Delft, Delft, Netherlands., Zadpoor AA; Department of Biomechanical Engineering, Faculty of Mechanical, Maritime and Materials Engineering, TU Delft, Delft, Netherlands., Fratila-Apachitei LE; Department of Biomechanical Engineering, Faculty of Mechanical, Maritime and Materials Engineering, TU Delft, Delft, Netherlands., Farrell E; Department of Oral and Maxillofacial Surgery, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2023 Jun 21; Vol. 11, pp. 1176534. Date of Electronic Publication: 2023 Jun 21 (Print Publication: 2023).
DOI:	10.3389/fbioe.2023.1176534
Abstrakt:	Macrophage responses following the implantation of orthopaedic implants are essential for successful implant integration in the body, partly through intimate crosstalk with human marrow stromal cells (hMSCs) in the process of new bone formation. Additive manufacturing (AM) and plasma electrolytic oxidation (PEO) in the presence of silver nanoparticles (AgNPs) are promising techniques to achieve multifunctional titanium implants. Their osteoimmunomodulatory properties are, however, not yet fully investigated. Here, we studied the effects of implants with AgNPs on human macrophages and the crosstalk between hMSCs and human macrophages when co-cultured in vitro with biofunctionalised AM Ti6Al4V implants. A concentration of 0.3 g/L AgNPs in the PEO electrolyte was found to be optimal for both macrophage viability and inhibition of bacteria growth. These specimens also caused a decrease of the macrophage tissue repair related factor C-C Motif Chemokine Ligand 18 (CCL18). Nevertheless, co-cultured hMSCs could osteogenically differentiate without any adverse effects caused by the presence of macrophages that were previously exposed to the PEO (±AgNPs) surfaces. Further evaluation of these promising implants in a bony in vivo environment with and without infection is highly recommended to prove their potential for clinical use. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Garmendia Urdalleta, Van Poll, Fahy, Witte-Bouma, Van Wamel, Apachitei, Zadpoor, Fratila-Apachitei and Farrell.)
Databáze:	MEDLINE
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