A simple preparation of prestained molecular markers for electrophoresis using inexpensive and readily available proteins.

Autor: Chubatsu LS; Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil. Electronic address: chubatsu@ufpr.br., Gerhardt ECM; Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil., Souza EM; Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2023 Sep 01; Vol. 676, pp. 115231. Date of Electronic Publication: 2023 Jul 04.
DOI: 10.1016/j.ab.2023.115231
Abstrakt: Protein electrophoresis in polyacrylamide gels in the presence of sodium dodecyl sulfate (SDS-PAGE) is one of the most commonly performed procedures in biochemical laboratories. It requires the use of molecular weight (MW) markers as an internal technical control and to determine the migration rate of a particular protein. In this work, we describe a simple method for preparing "homemade" prestained protein markers using readily available cow's milk and chicken egg white proteins without the need of any major protein purification step, and produce prestained MW markers ranging from 19 to 98 kDa.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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