Measurements of the Vitamin D Metabolome in the Calgary Vitamin D Study: Relationship of Vitamin D Metabolites to Bone Loss.

Autor: Burt LA; McCaig Institute for Bone and Joint Health, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada., Kaufmann M; Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada., Rose MS; Research Facilitation, Alberta Health Services, Calgary, Alberta, Canada., Jones G; Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada., Billington EO; McCaig Institute for Bone and Joint Health, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.; Division of Endocrinology & Metabolism, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada., Boyd SK; McCaig Institute for Bone and Joint Health, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada., Hanley DA; McCaig Institute for Bone and Joint Health, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.; Division of Endocrinology & Metabolism, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
Jazyk: angličtina
Zdroj: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research [J Bone Miner Res] 2023 Sep; Vol. 38 (9), pp. 1312-1321. Date of Electronic Publication: 2023 Jul 17.
DOI: 10.1002/jbmr.4876
Abstrakt: In a 36-month randomized controlled trial examining the effect of high-dose vitamin D 3 on radial and tibial total bone mineral density (TtBMD), measured by high-resolution peripheral quantitative tomography (HR-pQCT), participants (311 healthy males and females aged 55-70 years with dual-energy X-ray absorptiometry T-scores > -2.5 without vitamin D deficiency) were randomized to receive 400 IU (N = 109), 4000 IU (N = 100), or 10,000 IU (N = 102) daily. Participants had HR-pQCT radius and tibia scans and blood sampling at baseline, 6, 12, 24, and 36 months. This secondary analysis examined the effect of vitamin D dose on plasma measurements of the vitamin D metabolome by liquid chromatography-tandem mass spectrometry (LC-MS/MS), exploring whether the observed decline in TtBMD was associated with changes in four key metabolites [25-(OH)D 3 ; 24,25-(OH) 2 D 3 ; 1,25-(OH) 2 D 3 ; and 1,24,25-(OH) 3 D 3 ]. The relationship between peak values in vitamin D metabolites and changes in TtBMD over 36 months was assessed using linear regression, controlling for sex. Increasing vitamin D dose was associated with a marked increase in 25-(OH)D 3 , 24,25-(OH) 2 D 3 and 1,24,25-(OH) 3 D 3 , but no dose-related change in plasma 1,25-(OH) 2 D 3 was observed. There was a significant negative slope for radius TtBMD and 1,24,25-(OH) 3 D 3 (-0.05, 95% confidence interval [CI] -0.08, -0.03, p < 0.001) after controlling for sex. A significant interaction between TtBMD and sex was seen for 25-(OH)D 3 (female: -0.01, 95% CI -0.12, -0.07; male: -0.04, 95% CI -0.06, -0.01, p = 0.001) and 24,25-(OH) 2 D 3 (female: -0.75, 95% CI -0.98, -0.52; male: -0.35, 95% CI -0.59, -0.11, p < 0.001). For the tibia there was a significant negative slope for 25-(OH)D 3 (-0.03, 95% CI -0.05, -0.01, p < 0.001), 24,25-(OH) 2 D 3 (-0.30, 95% CI -0.44, -0.16, p < 0.001), and 1,24,25-(OH) 3 D 3 (-0.03, 95% CI -0.05, -0.01, p = 0.01) after controlling for sex. These results suggest vitamin D metabolites other than 1,25-(OH) 2 D 3 may be responsible for the bone loss seen in the Calgary Vitamin D Study. Although plasma 1,25-(OH) 2 D 3 did not change with vitamin D dose, it is possible rapid catabolism to 1,24,25-(OH) 3 D 3 prevented the detection of a dose-related rise in plasma 1,25-(OH) 2 D 3 . © 2023 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
(© 2023 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).)
Databáze: MEDLINE
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