Trichoderma koningiopsis (Hypocreaceae) has the smallest mitogenome of the genus Trichoderma .

Autor:	Castrillo ML; Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones 'Dra. María Ebe Reca'-InBioMis, Universidad Nacional de Misiones, Posadas, Misiones, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Bich GÁ; Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones 'Dra. María Ebe Reca'-InBioMis, Universidad Nacional de Misiones, Posadas, Misiones, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Amerio NS; Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones 'Dra. María Ebe Reca'-InBioMis, Universidad Nacional de Misiones, Posadas, Misiones, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Barengo MP; Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones 'Dra. María Ebe Reca'-InBioMis, Universidad Nacional de Misiones, Posadas, Misiones, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Zapata PD; Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones 'Dra. María Ebe Reca'-InBioMis, Universidad Nacional de Misiones, Posadas, Misiones, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Saparrat MCN; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.; Facultad de Ciencias Agrarias y Forestales, Instituto de Fisiología Vegetal, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.; Facultad de Ciencias Naturales y Museo, Instituto de Botánica Carlos Spegazzini, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.; Cátedra de Microbiología Agrícola, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina., Villalba LL; Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones 'Dra. María Ebe Reca'-InBioMis, Universidad Nacional de Misiones, Posadas, Misiones, Argentina.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 Jun 13; Vol. 14, pp. 1141087. Date of Electronic Publication: 2023 Jun 13 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1141087
Abstrakt:	Introduction: Fungal mitogenomes exhibit remarkable variation in conformation, size, gene content, arrangement and expression, including their intergenic spacers and introns. Methods: The complete mitochondrial genome sequence of the mycoparasitic fungus Trichoderma koningiopsis was determined using the Illumina next-generation sequencing technology. We used data from our recent Illumina NGS-based project of T. koningiopsis genome sequencing to study its mitochondrial genome. The mitogenome was assembled, annotated, and compared with other fungal mitogenomes. Results: T. koningiopsis strain POS7 mitogenome is a circular molecule of 27,560 bp long with a GC content of 27.80%. It harbors the whole complement of the 14 conserved mitochondrial protein-coding genes (PCG) such as atp 6, atp 8, atp 9, cox 1, cox 2, cox 3, cob , nad 1, nad 2, nad 3, nad 4, nad 4L, nad 5, and nad 6, also found in the same gene order to other Hypocreales. The mitogenome also contains 26 transfer RNA genes (tRNAs), 5 of them with more than one copy. Other genes also present in the assembled mitochondrial genome are a small rRNA subunit and a large rRNA subunit containing ribosomal protein S3 gene. Despite the small genome size, two introns were detected in the T. koningiopsis POS7 mitogenome, one of them in cox 3 gene and the other in rnl gene, accounting 7.34% of this mitogenome with a total size of 2,024 bp. A phylogenetic analysis was done using the 14 PCGs genes of T. koningiopsis strain POS7 mitogenome to compare them with those from other fungi of the Subphyla Pezizomycotina and Saccharomycotina. T. koningiopsis strain POS7 was clustered together with other representatives of Trichoderma lineage, within the Hypocreales group, which is also supported by previous phylogenetic studies based on nuclear markers. Discussion: The mitochondrial genome of T. koningiopsis POS7 will allow further investigations into the taxonomy, phylogenetics, conservation genetics, and evolutionary biology of this important genus as well as other closely related species. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Castrillo, Bich, Amerio, Barengo, Zapata, Saparrat and Villalba.)
Databáze:	MEDLINE
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