Akanthomyces diversity in Brazil and their pathogenicity to plant-sucking insects.

Autor: Lopes RB; Embrapa Genetic Resources and Biotechnology, Brasilia, Federal District, 70770-917, Brazil. Electronic address: rogerio.lopes@embrapa.br., Souza TAD; Federal University of Lavras, Department of Plant Pathology, Lavras, MG 37203-202, Brazil. Electronic address: tamires.souza1@estudante.ufla.br., Mascarin GM; Embrapa Environment, Road SP-340 Km 127.5, Jaguariuna, SP 13918-110, Brazil. Electronic address: gabriel.mascarin@embrapa.br., Souza DA; Embrapa Genetic Resources and Biotechnology, Brasilia, Federal District, 70770-917, Brazil. Electronic address: daniela.aguiar@embrapa.br., Bettiol W; Embrapa Environment, Road SP-340 Km 127.5, Jaguariuna, SP 13918-110, Brazil. Electronic address: wagner.bettiol@embrapa.br., Souza HR; Embrapa Genetic Resources and Biotechnology, Brasilia, Federal District, 70770-917, Brazil. Electronic address: hebertribeiro3@gmail.com., Faria M; Embrapa Genetic Resources and Biotechnology, Brasilia, Federal District, 70770-917, Brazil. Electronic address: marcos.faria@embrapa.br.
Jazyk: angličtina
Zdroj: Journal of invertebrate pathology [J Invertebr Pathol] 2023 Sep; Vol. 200, pp. 107955. Date of Electronic Publication: 2023 Jun 24.
DOI: 10.1016/j.jip.2023.107955
Abstrakt: Currently, species within the genus Akanthomyces are poorly studied and explored compared to other hypocrealean entomopathogenic fungi employed as commercial biocontrol agents. This study aimed to molecularly identify 23 Brazilian Akanthomyces strains, most originally isolated from aphids and scales (n = 22), and one from the coffee leaf rust, and further investigate their pathogenicity to six plant-sucking insects as a means to better understand their host spectra. We also explored the capacity of A. muscarius CG935 for blastospore production via liquid fermentation. Akanthomyces dipterigenus, A. muscarius, A. lecanii, and two unidentified species were recognized as naturally occurring in Brazil. Akanthomyces dipterigenus CG829 and A. muscarius CG935 were highly virulent to nymphs of Bemisia tabaci (67.5-85.4% confirmed mortality) and the aphid Aphis fabae (74.6-75.3%), but only the first strain was virulent to the mealybug Planococcus sp. (80.9%). Akanthomyces lecanii CG824 was weakly virulent to all tested insects. None of the strains were pathogenic to the thrips Caliothrips phaseoli, and all strains showed low virulence to the wooly whitefly Aleurothrixus floccosus and the scale Duplachionaspis divergens. Submerged liquid fermentation yields varied from 1.72 × 10 9 (day 2) to 3.90 × 10 9 (day 5) blastospores mL -1 . Blastospores or aerial conidia from A. muscarius CG935, at a single concentration of 1 × 10 7 viable propagules mL -1 , resulted in 67.5-83.1% mortality of B. tabaci nymphs within 8 days post-treatment. Overall, these results encourage additional studies that could lead to the development of new mycopesticides based on Akanthomyces strains.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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