Decreasing mRNA HMGB1 expression in Klebsiella pneumoniae infection treated by Miana ( Coleus scutellarioides (L.) Benth): a cohort experimental study.

Autor:	Akaputra R; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta, Jakarta., Hatta M; Department of Molecular Biology and Immunology., Massi MN; Department of Molecular Biology and Immunology., Djaharuddin I; Department of Pulmonology and Respiratory Medicine., Bukhari A; Department of Nutrition., Aminuddin A; Department of Nutrition., Santoso A; Department of Pulmonology and Respiratory Medicine., Natzir R; Department of Biochemistry., Bahar B; Department of Biostatistic., Fachri M; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta, Jakarta., Farsida F; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta, Jakarta., Fathimah A; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta, Jakarta., Ubaidah FM; Department of Pulmonology and Respiratory Medicine, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta, Jakarta., Sridiana E; Postgraduate School., Dwiyanti R; Department of Medical Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia.; Department of Forensic and Medicolegal., Syukri A; Department of Molecular Biology and Immunology.; Postgraduate School., Junita AR; Department of Molecular Biology and Immunology.; Postgraduate School., Febrianti A; Department of Forensic and Medicolegal., Primaguna MR; Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar., Azhar A; Department of Pulmonology and Respiratory Medicine.
Jazyk:	angličtina
Zdroj:	Annals of medicine and surgery (2012) [Ann Med Surg (Lond)] 2023 May 23; Vol. 85 (6), pp. 2395-2399. Date of Electronic Publication: 2023 May 23 (Print Publication: 2023).
DOI:	10.1097/MS9.0000000000000908
Abstrakt:	Pneumonia is one of the most common infections caused by the bacterium Klebsiella pneumoniae . During the initiation of an infection, the immune system recognizes the pathogen through the release of high mobility group box 1 (HMGB1), thereby triggering the inflammation process. Miana has demonstrated potent inhibitory effects on the inflammatory process during infection in animal models. The aim of this study was to determine the effect of Miana leaf extract on mRNA HMGB1 expression in Balb/c mice infected with K. pneumoniae . Methods: This study comprised a cohort experiment using 20 Balb/c mice divided into four groups. Balb/c mice in each group were intraperitoneally injected with K. pneumoniae . Group 1 was given a placebo; Group 2 was given Miana; Group 3 was given levofloxacin; and Group 4 was given both levofloxacin and Miana. The levels of mRNA HMGB1 expression were measured using real-time PCR before, during, and after the infection as well as after the treatments. Results: The initial examination results showed that the average level of mRNA HMGB1 expression was 5.51 fc. The mRNA HMGB1 expression in mice after being challenged with K. pneumoniae was 9.64 fc. Group 1 that was given a placebo had a mean mRNA HMGB1 expression level of 14.99 fc. Group 2 that was given Miana had a mean mRNA HMGB1 expression level of 13.95 fc. Group 3 that was given levofloxacin had an average mRNA HMGB1 expression level of 6.45 fc, and Group 4 that was given levofloxacin and Miana together had an average mRNA HMGB1 expression level of 5.59 fc. Conclusion: Miana ( Coleus scutellarioides (L.) Benth) increased mRNA HMGB1 expression at the initial administration via regulation of the immune system. Administration of Miana following K. pneumoniae infection inhibited the increase in mRNA HMGB1 expression. Treatment with levofloxacin reduced the level of mRNA HMGB1 expression, and the effect was optimized by the administration of Miana leaf extract as a supplement. Competing Interests: The authors declare that there are no conflicts of interest, financial or otherwise. (Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.)
Databáze:	MEDLINE
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