Antibacterial polypeptide-bioparticle for oral administration: Powder formulation, palatability and in vivo toxicity approach.

Autor: Ramirez CAB; Laboratory of Nano Bio Materials, Department of Biophysics, Paulista Medical Scholl, Federal University of Sao Paulo, 04023-062 Sao Paulo, Brazil., Mathews PD; Laboratory of Nano Bio Materials, Department of Biophysics, Paulista Medical Scholl, Federal University of Sao Paulo, 04023-062 Sao Paulo, Brazil; Muséum National d'Histoire Naturelle, Sorbonne Université, CP26, 75231 Paris, France. Electronic address: patrickmathews83@gmail.com., Madrid RRM; Laboratory of Nano Bio Materials, Department of Biophysics, Paulista Medical Scholl, Federal University of Sao Paulo, 04023-062 Sao Paulo, Brazil., Garcia ITS; Department of Physical-Chemistry, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, Brazil., Rigoni VLS; Laboratory of Nano Bio Materials, Department of Biophysics, Paulista Medical Scholl, Federal University of Sao Paulo, 04023-062 Sao Paulo, Brazil., Mertins O; Laboratory of Nano Bio Materials, Department of Biophysics, Paulista Medical Scholl, Federal University of Sao Paulo, 04023-062 Sao Paulo, Brazil. Electronic address: mertins@unifesp.br.
Jazyk: angličtina
Zdroj: Biomaterials advances [Biomater Adv] 2023 Oct; Vol. 153, pp. 213525. Date of Electronic Publication: 2023 Jun 16.
DOI: 10.1016/j.bioadv.2023.213525
Abstrakt: The upsurge of bacterial resistance to conventional antibiotics turned a well-recognized public health threat. The need of developing new biomaterials of effective practical use in order to tackle bacterial resistance became urgent. In this study, a submicrometric bioparticle of known antibacterial activity was produced in powder form with suitable texture and appealing characteristics for effective oral administration. Through complex coacervating a natural-source antimicrobial polypeptide with chitosan-N-arginine and alginate, the bioactive polypeptide was physically incorporated to the bioparticle whose structure positively responds to the pH variations found in gastrointestinal tract. The powder formulation presented high palatability that was evaluated using fish as in vivo animal model. A thorough survey of the fish intestinal tissues, following a systematic oral administration, revealed high penetration potential of the biomaterial through epithelial cells and deeper intestine layers. Despite, no cytotoxic effect was observed in analyzing the tissues through different histology methods. The absence of intestinal damage was corroborated by immune histochemistry, being the integrity of epithelial motor myosin Vb and related traffic proteins preserved. Hematology further endorsed absence of toxicity in blood cells whose morphology was evaluated in detail. The study evidenced the applicability potential of a new biomaterial of appealing and safe oral administration of antibacterial polypeptide.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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