Hypoxia induced responses are reflected in the stromal proteome of breast cancer.

Autor: Kjølle S; Centre for Cancer Biomarkers CCBIO, Department of Clinical Medicine, Section for Pathology, University of Bergen, Bergen, N-5021, Norway., Finne K; Centre for Cancer Biomarkers CCBIO, Department of Clinical Medicine, Section for Pathology, University of Bergen, Bergen, N-5021, Norway., Birkeland E; Centre for Cancer Biomarkers CCBIO, Department of Clinical Medicine, Section for Pathology, University of Bergen, Bergen, N-5021, Norway., Ardawatia V; Centre for Cancer Biomarkers CCBIO, Department of Clinical Medicine, Section for Pathology, University of Bergen, Bergen, N-5021, Norway., Winge I; Centre for Cancer Biomarkers CCBIO, Department of Clinical Medicine, Section for Pathology, University of Bergen, Bergen, N-5021, Norway., Aziz S; Centre for Cancer Biomarkers CCBIO, Department of Clinical Medicine, Section for Pathology, University of Bergen, Bergen, N-5021, Norway.; Department of Pathology, Haukeland University Hospital, Bergen, N-5021, Norway., Knutsvik G; Centre for Cancer Biomarkers CCBIO, Department of Clinical Medicine, Section for Pathology, University of Bergen, Bergen, N-5021, Norway.; Department of Pathology, Haukeland University Hospital, Bergen, N-5021, Norway., Wik E; Centre for Cancer Biomarkers CCBIO, Department of Clinical Medicine, Section for Pathology, University of Bergen, Bergen, N-5021, Norway.; Department of Pathology, Haukeland University Hospital, Bergen, N-5021, Norway., Paulo JA; Department of Cell Biology, Harvard Medical School, Boston, MA, USA., Vethe H; Centre for Cancer Biomarkers CCBIO, Department of Clinical Medicine, Section for Pathology, University of Bergen, Bergen, N-5021, Norway., Kleftogiannis D; Centre for Cancer Biomarkers CCBIO, Department of Clinical Medicine, Section for Pathology, University of Bergen, Bergen, N-5021, Norway.; Department of Informatics, Computational Biology Unit, University of Bergen, Bergen, Norway., Akslen LA; Centre for Cancer Biomarkers CCBIO, Department of Clinical Medicine, Section for Pathology, University of Bergen, Bergen, N-5021, Norway. lars.akslen@uib.no.; Department of Pathology, Haukeland University Hospital, Bergen, N-5021, Norway. lars.akslen@uib.no.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Jun 22; Vol. 14 (1), pp. 3724. Date of Electronic Publication: 2023 Jun 22.
DOI: 10.1038/s41467-023-39287-7
Abstrakt: Cancers are often associated with hypoxia and metabolic reprogramming, resulting in enhanced tumor progression. Here, we aim to study breast cancer hypoxia responses, focusing on secreted proteins from low-grade (luminal-like) and high-grade (basal-like) cell lines before and after hypoxia. We examine the overlap between proteomics data from secretome analysis and laser microdissected human breast cancer stroma, and we identify a 33-protein stromal-based hypoxia profile (33P) capturing differences between luminal-like and basal-like tumors. The 33P signature is associated with metabolic differences and other adaptations following hypoxia. We observe that mRNA values for 33P predict patient survival independently of molecular subtypes and basic prognostic factors, also among low-grade luminal-like tumors. We find a significant prognostic interaction between 33P and radiation therapy.
(© 2023. The Author(s).)
Databáze: MEDLINE
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