Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate Cancer.

Autor: Saitta C; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy.; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy., De Simone I; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy., Fasulo V; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy.; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy., Corbetta M; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy., Duga S; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy.; Flow Cytometry Core, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy., Chiereghin C; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy., Colombo FS; Flow Cytometry Core, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy., Benetti A; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy., Contieri R; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy.; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy., Avolio PP; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy.; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy., Uleri A; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy.; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy., Saita A; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy., Guazzoni GF; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy.; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy., Hurle R; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy., Colombo P; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy.; Department of Pathology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy., Buffi NM; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy.; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy., Casale P; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy., Lughezzani G; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy.; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy., Asselta R; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy.; Flow Cytometry Core, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy., Soldà G; Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy.; Flow Cytometry Core, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy., Lazzeri M; Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy.
Jazyk: angličtina
Zdroj: Cancers [Cancers (Basel)] 2023 May 09; Vol. 15 (10). Date of Electronic Publication: 2023 May 09.
DOI: 10.3390/cancers15102666
Abstrakt: Liquid biopsy (LB) for prostate cancer (PCa) detection could represent an alternative to biopsy. Seminal fluid (SF) is a source of PCa-specific biomarkers, as 40% of ejaculate derives from the prostate. We tested the feasibility of an SF-based LB by evaluating the yield of semen self-sampling in a cohort of >750 patients with clinically localized PCa. The overall SF collection yield was 18.2% (39% when considering only compliant patients), with about a half of the patients (53.15%) not consenting to SF donation. Independent favorable predictors for SF collection were younger age and lower prostate volume. We implemented a protocol to enrich prostate-derived cells by multi-color flow cytometry and applied it on SF and urine samples from 100 patients. The number of prostate-enriched cells (SYTO-16+ PSMA+ CD45-) was variable, with higher numbers of cells isolated from SF than urine ( p value < 0.001). Putative cancer cells (EpCAM high ) were 2% of isolated cells in both specimens. The fraction of EpCAM high cells over prostate-enriched cells (PSMA+) significantly correlated with patient age in both semen and urine, but not with other clinical parameters, such as Gleason Score, ISUP, or TNM stage. Hence, enumeration of prostate-derived cells is not sufficient to guide PCa diagnosis; additional molecular analyses to detect patient-specific cancer lesions will be needed.
Databáze: MEDLINE
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