Silver Nitrate Staining of Nucleolar Organizer Regions (Ag-NORs) in Plant Chromosomes.

Autor:	Palma-Rojas C; Departamento de Biología, Universidad de La Serena, La Serena, Chile., Jara-Seguel P; Departamento de Ciencias Biológicas y Químicas, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile. pjara@uct.cl.; Núcleo de Estudios Ambientales, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile. pjara@uct.cl., Araya-Jaime C; Departamento de Biología, Universidad de La Serena, La Serena, Chile.; Instituto de Investigación Multidisciplinario de Investigación y postgrado. Universidad de La Serena, La Serena, Chile.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2672, pp. 225-231.
DOI:	10.1007/978-1-0716-3226-0_13
Abstrakt:	Silver nitrate staining to evidence the location of nucleolar organizer regions (Ag-NORs) in chromosomes is widely used as a classical method in plant cytogenetics. Here, we present the most used procedures and highlight some aspects in terms of their replicability by plant cytogeneticists. Some technical features described are materials and methods used, procedures, protocol modifications, and precautions in order to obtain positive signals. The methods to obtain Ag-NOR signals have different degrees of replicability, but do not require any sophisticated technology or equipment for their application. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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