Crude saliva of Amblyomma cajennense sensu stricto (Acari: Ixodidae) reduces locomotor activity and increases the hemocyte number in the females of Aedes aegypti (Diptera: Culicidae).

Autor: Cerri F; Laboratório de Neuro e Imunofarmacologia (NIMFAR), Fundação Oswaldo Cruz (Fiocruz), Fiocruz Rondônia, Porto Velho, RO, Brasil; Programa de Pós-Graduação em Biologia Experimental (PGBIOEXP), Universidade Federal de Rondônia (UNIR), Porto Velho, RO, Brasil., Araujo MDS; Laboratório de Entomologia/Plataforma de Produção e Infecção de Vetores da Malária (PIVEM) - Fundação Oswaldo Cruz (Fiocruz), Fiocruz Rondônia, Porto Velho, RO, Brasil., Aguirre AAR; Laboratório de Entomologia/Plataforma de Produção e Infecção de Vetores da Malária (PIVEM) - Fundação Oswaldo Cruz (Fiocruz), Fiocruz Rondônia, Porto Velho, RO, Brasil; Plataforma de Criação e Experimentação Animal (PCEA), Fundação Oswaldo Cruz (Fiocruz), Fiocruz Rondônia, Porto Velho, RO, Brasil., Evaristo GPC; Centro de Estudos de Biomoléculas Aplicadas à Saúde (CEBio), Fundação Oswaldo Cruz (Fiocruz), Fiocruz Rondônia, Porto Velho, RO, Brasil., Evaristo JAM; Laboratório de Proteômica (LADETEC), Instituto de Química (IQ), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brasil., Nogueira FCS; Laboratório de Proteômica (LADETEC), Instituto de Química (IQ), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brasil., de Medeiros JF; Programa de Pós-Graduação em Biologia Experimental (PGBIOEXP), Universidade Federal de Rondônia (UNIR), Porto Velho, RO, Brasil; Laboratório de Entomologia/Plataforma de Produção e Infecção de Vetores da Malária (PIVEM) - Fundação Oswaldo Cruz (Fiocruz), Fiocruz Rondônia, Porto Velho, RO, Brasil., Dias QM; Laboratório de Neuro e Imunofarmacologia (NIMFAR), Fundação Oswaldo Cruz (Fiocruz), Fiocruz Rondônia, Porto Velho, RO, Brasil; Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação (INCT - NIM), Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil; Programa de Pós-Graduação em Biologia Experimental (PGBIOEXP), Universidade Federal de Rondônia (UNIR), Porto Velho, RO, Brasil; Centro Unuversitário São Lucas (UNISL) - Afya, Porto Velho, RO, Brasil. Electronic address: quintino.dias@fiocruz.br.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2023 Aug; Vol. 251, pp. 108570. Date of Electronic Publication: 2023 Jun 15.
DOI: 10.1016/j.exppara.2023.108570
Abstrakt: Aedes aegypti are vector insects of arboviruses such as dengue, Zika, and chikungunya. All available vector control methods have limited efficacy, highlighting the urgent need to find alternative ones. Evidence shows that arachnids like ticks are sources of biologically active compounds. Moreover, chemical modulation of the locomotor and immune systems of vector insects can be used to control arbovirus transmission. The present study evaluated the effectiveness of crude saliva of female Amblyomma cajennense sensu stricto (s.s.) ticks in reducing locomotor activity and inducing an immune response in Ae. aegypti females. Additionally, the study evaluated the protein constitution of tick saliva. For this purpose, the crude saliva obtained from several semi-engorged A. cajennense females was used. A volume of 0.2 nL of crude tick saliva was administered to mosquitoes by direct intrathoracic microinjection. The effect of the tick's saliva on the locomotor activity of the mosquito was observed using Flybox, a video-automated monitoring system, and the hemolymph hemocyte levels were quantified by reading slides under a light microscope. The protein concentration of the crude tick saliva was 1.27 μg/μL, and its electrophoretic profile indicates the presence of proteins with a molecular weight ranging between ∼17 and 95 kDa. Microplusins, ixodegrins, cystatin, actins, beta-actin, calponin, albumin, alpha-globulins, and hemoglobin were the main proteins identified by proteomics in the saliva of A. cajennense. The microinjected saliva had low toxicity for Ae. aegypti females and significantly reduced their locomotor activity, especially in the transition between the light and dark phases. The crude tick saliva did not change the period and rhythmicity of the circadian cycle. The tick saliva significantly increased the number of hemocytes two days after injection and reduced it after five days. These results suggest that further evaluation of the biological properties of tick saliva proteins against Ae. aegypti would be of interest.
Competing Interests: Declaration of competing interest All authors disclose some financial and personal relationships with other people or organizations that could inappropriately influence in this work. The authors declare no conflict of interest.
(Published by Elsevier Inc.)
Databáze: MEDLINE
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