Extraction, identification and antitumor activity in cell cultures of cubebin isolated from dry seeds Piper cubeba (Piperaceae).

Autor: Costa E Silva Scarpa Pereira C; Faculty of Pharmaceutical Sciences, University of Campinas, Cidade Universitária Zeferino Vaz, 13081-970, Campinas, SP, Brazil., Perazzo FF; Department of Exact Sciences and Earth, Federal University of São Paulo, Rua Arthur Riedel 275, Jardim Eldorado, 09072-270, Diadema, São Paulo, Brazil., Bianco de Souza GH; Department of Pharmacy, School of Pharmacy, University Federal of Ouro Preto, Campus Morro do Cruzeiro s/n, Bauxita, 35400-000, Ouro Preto, Minas Gerais, Brazil., Affonso Fonseca FL; Department of Exact Sciences and Earth, Federal University of São Paulo, Rua Arthur Riedel 275, Jardim Eldorado, 09072-270, Diadema, São Paulo, Brazil; ABC Foundation Schol of Medicine, Santo André, São Paulo, Brazil., Bonilha Dezena RM; Preformulation Specialist Consultant, Campinas, São Paulo, Brazil., Gois Ruiz ALT; Faculty of Pharmaceutical Sciences, University of Campinas, Cidade Universitária Zeferino Vaz, 13081-970, Campinas, SP, Brazil., Pires Rosa PC; Faculty of Pharmaceutical Sciences, University of Campinas, Cidade Universitária Zeferino Vaz, 13081-970, Campinas, SP, Brazil. Electronic address: paulocpr@unicamp.br.
Jazyk: angličtina
Zdroj: Annales pharmaceutiques francaises [Ann Pharm Fr] 2023 Nov; Vol. 81 (6), pp. 950-967. Date of Electronic Publication: 2023 Jun 15.
DOI: 10.1016/j.pharma.2023.06.002
Abstrakt: The Pipercubeba, it is one spice, widely consumed in Europe, which has several bioactive molecules, between those a lignan named cubebin. Cubebin has several known biological activities, such as analgesic activity and anti-inflammatory, trypanocidal activity, leishmanicidal and antitumor activity. The objective of this study was to evaluate the antiproliferative activity "in vitro" cubebin in eight different human tumor cell lines. It was fully characterized by IR analysis, NMR, mass spectrometry, DSC, TGA, residual solvent and elemental analysis. The antitumor activity of cubebin was evaluated "in vitro" on eight different human tumor cell lineages. Cubebin showed GI 50 ≤30μg/mL for lineage cell U251 (glioma CNS), 786-0 (kidney), PC-3 (prostate), HT-29 (colon rectum). For K562 cells (leukemia), cubebin presented GI 50 ≤to 4.0mg/mL. For the other lineages cells, MCF-7 (breast) and NCI-H460 to cubebin can be considered inactive because of GI 50 >250mg/mL. Analyzing the selectivity index for cubebin, it can be observed that high selectivity of cubebin to K562 lineage cells (leukemia). Analyzing the cytotoxic potential of cubebin was observed that probably acts cubebin altering metabolism, inhibiting cell growth - a cytostatic effect, showing no cytocidal effect on any lineage cell.
(Copyright © 2023 Académie Nationale de Pharmacie. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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