The impact of xylan on the biosynthesis and structure of extracellular lignin produced by a Norway spruce tissue culture.

Autor: Sapouna I; Wallenberg Wood Science Center KTH Royal Institute of Technology Stockholm Sweden.; Division of Glycoscience Department of Chemistry KTH Royal Institute of Technology AlbaNova University Center Stockholm Sweden., Kärkönen A; Production Systems Natural Resources Institute Finland (Luke) Helsinki Finland.; Viikki Plant Science Centre Department of Agricultural Sciences University of Helsinki Helsinki Finland., McKee LS; Wallenberg Wood Science Center KTH Royal Institute of Technology Stockholm Sweden.; Division of Glycoscience Department of Chemistry KTH Royal Institute of Technology AlbaNova University Center Stockholm Sweden.
Jazyk: angličtina
Zdroj: Plant direct [Plant Direct] 2023 Jun 12; Vol. 7 (6), pp. e500. Date of Electronic Publication: 2023 Jun 12 (Print Publication: 2023).
DOI: 10.1002/pld3.500
Abstrakt: In order to develop more economic uses of lignin, greater knowledge regarding its native structure is required. This can inform the development of optimized extraction methods that preserve desired structural properties. Current extraction methods alter the polymeric structure of lignin, leading to a loss of valuable structural groups or the formation of new non-native ones. In this study, Norway spruce ( Picea abies ) tissue-cultured cells that produce lignin extracellularly in a suspension medium were employed. This system enables the investigation of unaltered native lignin, as no physicochemical extraction steps are required. For the first time, this culture was used to investigate the interactions between lignin and xylan, a secondary cell wall hemicellulose, and to study the importance of lignin-carbohydrate complexes (LCCs) on the polymerization and final structure of extracellular lignin (ECL). This has enabled us to study the impact of xylan on monolignol composition and structure of the final lignin polymer. We find that the addition of xylan to the solid culture medium accelerates cell growth and impacts the ratio of monolignols in the lignin. However, the presence of xylan in the lignin polymerization environment does not significantly alter the structural properties of lignin as analyzed by two-dimensional nuclear magnetic resonance (NMR) spectroscopy and size exclusion chromatography (SEC). Nevertheless, our data indicate that xylan can act as a nucleation point, leading to more rapid lignin polymerization, an important insight into biopolymer interactions during cell wall synthesis in wood. Lignin structure and interactions with a secondary cell wall hemicellulose were investigated in a model cell culture: we found that the polymerization and final structure of lignin are altered when the hemicellulose is present during cell growth and monolignol production. The physicochemical interactions between lignin and xylan partly define the extractability and utility of native lignin in high value applications, so this work has implications for lignin extraction as well as fundamental plant biology.
Competing Interests: The authors declare no conflicts of interest.
(© 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: