| Autor: |
Ball M; Sant Joan de Déu Research Institute; Vanderbilt University School of Medicine, Vanderbilt University., Benito S; Sant Joan de Déu Research Institute; Department of Pediatric Critical Care Medicine, Sant Joan de Déu Hospital., Caride JP; Sant Joan de Déu Research Institute., Ruiz-Herguido C; Sant Joan de Déu Research Institute., Camprubí-Camprubí M; Sant Joan de Déu Research Institute; BCNatal-Barcelona Center for Maternal Fetal and Neonatal Medicine, Sant Joan de Déu Hospital; marta.camprubi@sjd.es., Sanchez-de-Toledo J; Sant Joan de Déu Research Institute; Department of Pediatric Cardiology, Sant Joan de Déu Hospital; Department of Critical Care Medicine, University of Pittsburgh. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2023 May 26 (195). Date of Electronic Publication: 2023 May 26. |
| DOI: |
10.3791/65266 |
| Abstrakt: |
Congenital heart disease (CHD) is the most prevalent congenital malformation, with about one million births impacted worldwide per year. Comprehensive investigation of this disease requires appropriate and validated animal models. Piglets are commonly used for translational research due to their analogous anatomy and physiology. This work aimed to describe and validate a neonatal piglet model of cardiopulmonary bypass (CPB) with circulatory and cardiac arrest (CA) as a tool for studying severe brain damage and other complications of cardiac surgery. In addition to including a list of materials, this work provides a roadmap for other investigators to plan and execute this protocol. After experienced practitioners performed several trials, the representative results of the model demonstrated a 92% success rate, with failures attributed to small piglet size and variant vessel anatomy. Furthermore, the model allowed practitioners to select from a wide variety of experimental conditions, including varying times in CA, temperature alterations, and pharmacologic interventions. In summary, this method uses materials readily available in most hospital settings, is reliable and reproducible, and can be widely employed to enhance translational research in children undergoing heart surgery. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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