Measuring Neuronal Network Activity Using Human Induced Neuronal Cells.

Autor:	McSweeney D; Graduate Program in Molecular and Cellular Biology, University of Massachusetts Amherst, Amherst, MA, USA.; Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA, USA., English J; Graduate Program in Molecular and Cellular Biology, University of Massachusetts Amherst, Amherst, MA, USA.; Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA, USA., Howell E; Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA, USA., Ribbe F; Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA, USA., Pak C; Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA, USA. cpak@umass.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2683, pp. 235-245.
DOI:	10.1007/978-1-0716-3287-1_19
Abstrakt:	Synchronous firing of neurons, often referred to as "network activity" or "network bursting," is an indication of a mature and synaptically connected network of neurons. We previously reported this phenomenon in 2D human neuronal in vitro models (McSweeney et al. iScience 25:105187, 2022). Using induced neurons (iNs) differentiated from human pluripotent stem cells (hPSCs) coupled with high-density microelectrodes arrays (HD-MEAs), we probed the underlying patterns of neuronal activity and found irregularities in network signaling across mutant states (McSweeney et al. iScience 25:105187, 2022). Here, we describe methods for plating cortical excitatory iNs differentiated from hPSCs on top of HD-MEAs and culturing iNs to maturity, examples of representative human wild-type Ngn2-iN data, and troubleshooting tips and tricks for the experimenter interested in integrating HD-MEAs into one's research approach. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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