Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation.

Autor:	Stephens DC; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA., Mungai M; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA., Crabtree A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Garza-Lopez E; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA., Vang L; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Neikirk K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Vue Z; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Vue N; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Marshall AG; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Turner K; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA., Shao JQ; Central Microscopy Research Facility, University of Iowa, Iowa City, IA, 52242, USA., Sarker B; School of Applied Computational Sciences, Meharry Medical College, Nashville, TN, 37232, USA., Murray S; Department of Cell Biology, College of Medicine, University of Pittsburgh, Pittsburgh, TN, 15260, USA., Gaddy JA; Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.; Tennessee Valley Healthcare Systems, U.S. Department of Veterans Affairs, Nashville, Tennessee, USA., Davis J; Department of Biochemistry and Cancer Biology. Meharry Medical College, Nashville, TN, USA., Damo SM; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA., Hinton AO Jr; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Aug 19. Date of Electronic Publication: 2023 Aug 19.
DOI:	10.1101/2023.05.20.541600
Abstrakt:	Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many cellular populations and validation of these populations is highly important. Therefore, in this article, we discuss a comprehensive method to isolate mice skeletal muscle, create satellite cells for tissue culture, and use immunofluorescence to validate our approach. Competing Interests: All authors have no competing interests. This project was funded by the UNCF/Bristol-Myers Squibb E.E. Just Faculty Fund, BWF Career Awards at the Scientific Interface Award, BWF Ad-hoc Award, NIH Small Research Pilot Subaward to 5R25HL106365-12 from the National Institutes of Health PRIDE Program, DK020593, Vanderbilt Diabetes and Research Training Center for DRTC Alzheimer’s Disease Pilot & Feasibility Program. CZI Science Diversity Leadership grant number 2022- 253529 from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (to A.H.J.). NSF EES2112556, NSF EES1817282, and CZI Science Diversity Leadership grant number 2022-253614 from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation (to S.M. D.) and National Institutes of Health grant HD090061 and the Department of Veterans Affairs Office of Research award I01 BX005352 (to J.G.). Additional support was provided by the Vanderbilt Institute for Clinical and Translational Research program supported by the National Center for Research Resources, Grant UL1 RR024975–01, and the National Center for Advancing Translational Sciences, Grant 2 UL1 TR000445–06 and the Cell Imaging Shared Resource. BioRender was used for the creation of Figures.
Databáze:	MEDLINE
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