Effect of bromelain on periodontal destruction and alveolar bone in rats with experimental periodontitis.

Autor: Paksoy T; Department of Periodontology, Hamidiye Faculty of Dentistry, University of Health Sciences, Istanbul, Turkey. Electronic address: tugceakap86@hotmail.com., Ustaoğlu G; Department of Periodontology, Faculty of Gülhane Dentistry, University of Health Sciences, Ankara, Turkey., Şehirli AÖ; Department of Pharmacology, Faculty of Dentistry, Near East University, 99138 Nicosia, Northern Cyprus, Mersin 10, Turkey., Ünsal RBK; Department of Periodontology, Faculty of Dentistry, University of Kyrenia, 99320 Kyrenia, Northern Cyprus, Mersin 10, Turkey., Sayıner S; Department of Biochemistry, Faculty of Veterinary Medicine, Near East University, 99138 Nicosia, Northern Cyprus, Mersin 10, Turkey., Orhan K; Department of DentoMaxillofacial Radiology, Ankara University, Ankara, Turkey., Aycı NB; Department of Histology and Embryology, School of Medicine, Tekirdağ Namık Kemal University, Tekirdağ, Turkey., Çetinel Ş; Department of Histology and Embryology, School of Medicine, Marmara University, Istanbul, Turkey., Aksoy U; Department of Endodontics, Faculty of Dentistry, Near East University, 99138 Nicosia, Northern Cyprus, Mersin 10, Turkey., Öğünç AV; Vocational School of Health-Related Professions, Marmara University, Istanbul, Turkey.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2023 Aug; Vol. 121, pp. 110446. Date of Electronic Publication: 2023 Jun 07.
DOI: 10.1016/j.intimp.2023.110446
Abstrakt: Purpose: Several substances that have anti-inflammatory, antiproteinase, and anti-infective properties have been evaluated as modulators of the inflammatory response in periodontal disease. However, evidence for the anti-inflammatory and antioxidative activities of bromelain is limited. This study evaluated the impact of systemically administered bromelain on the progression of experimental periodontitis.
Methods: Four equal groups of 32 Wistar albino rats were created as follows (n = 8): control, periodontitis + saline, periodontitis + 5 mg/kg/day bromelain, and periodontitis + 10 mg/kg/day bromelain. To quantify the resorption of bone and bone volume/tissue volume, bone surface / bone volume, and connectivity, lower jawbones were fixed and then scanned using microcomputed tomography (micro CT). Blood samples were taken to measure the macrophage colony-stimulating factor(M-CSF) concentrations, receptor activator of nuclear factor kappa-Β ligand (RANKL), osteoprotegerin (OPG), tumor necrosis factor-alpha (TNF-α), matrix metalloproteinase-8 (MMP-8), interleukin-6(IL-6), glutathione peroxidase (GPx), superoxide dismutase (SOD), and malondialdehyde (MDA). Histopathological assessments were made to examine the tissue.
Results: Treatment with bromelain improved the healing of the periodontium by decreasing the number of leukocytes and ligament deterioration in the gingival connective tissue and by supporting reintegration with alveolar bone. Bromelain used in ligature-induced periodontitis reduced alveolar bone (AB) resorption as measured by microCT; reduced inflammatory parameters such as IL-6 and TNF-α; regulated oxidative-antioxidative processes by increasing GPx and SOD and reducing MDA levels; and regulated AB modeling by decreasing M-CSF, RANKL, and MMP-8 and increasing OPG levels.
Conclusion: Bromelain may be an option in periodontal therapy by regulating cytokine levels, improving the healing process, and reducing bone resorption and oxidative stress.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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