Associations of gut microbiome with endogenous estrogen levels in healthy postmenopausal women.

Autor: Yaghjyan L; Department of Epidemiology, College of Public Health and Health Professions and College of Medicine, University of Florida, 2004 Mowry Rd, Gainesville, FL, 32610, USA. lyaghjyan@ufl.edu., Mai V; Department of Epidemiology, College of Public Health and Health Professions and College of Medicine, University of Florida, 2004 Mowry Rd, Gainesville, FL, 32610, USA.; Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA., Darville LNF; H. Lee Moffitt Cancer Center, Tampa, FL, USA., Cline J; H. Lee Moffitt Cancer Center, Tampa, FL, USA., Wang X; H. Lee Moffitt Cancer Center, Tampa, FL, USA., Ukhanova M; Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA., Tagliamonte MS; Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA.; Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, USA., Martinez YC; H. Lee Moffitt Cancer Center, Tampa, FL, USA., Rich SN; Department of Epidemiology, College of Public Health and Health Professions and College of Medicine, University of Florida, 2004 Mowry Rd, Gainesville, FL, 32610, USA., Koomen JM; H. Lee Moffitt Cancer Center, Tampa, FL, USA., Egan KM; H. Lee Moffitt Cancer Center, Tampa, FL, USA.
Jazyk: angličtina
Zdroj: Cancer causes & control : CCC [Cancer Causes Control] 2023 Oct; Vol. 34 (10), pp. 873-881. Date of Electronic Publication: 2023 Jun 07.
DOI: 10.1007/s10552-023-01728-5
Abstrakt: Purpose: The gut microbiome is a potentially important contributor to endogenous estrogen levels after menopause. In healthy postmenopausal women, we examined associations of fecal microbiome composition with levels of urinary estrogens, their metabolites, and relevant metabolic pathway ratios implicated in breast cancer risk.
Methods: Eligible postmenopausal women (n = 164) had a body mass index (BMI) ≤ 35 kg/m 2 and no history of hormone use (previous 6 months) or cancer/metabolic disorders. Estrogens were quantified in spot urine samples with liquid chromatography-high resolution mass spectrometry (corrected for creatinine). Bacterial DNA was isolated from fecal samples and the V1-V2 hypervariable regions of 16S rRNA were sequenced on the Illumina MiSeq platform. We examined associations of gut microbiome's indices of within-sample (alpha) diversity (i.e., Shannon, Chao1, and Inverse Simpson), phylogenetic diversity, and the ratio of the two main phyla (Firmicutes and Bacteroidetes; F/B ratio) with individual estrogens and metabolic ratios, adjusted for age and BMI.
Results: In this sample of 164 healthy postmenopausal women, the mean age was 62.9 years (range 47.0-86.0). We found significant inverse associations of observed species with 4-pathway:total estrogens (p = 0.04) and 4-pathway:2-pathway (p = 0.01). Shannon index was positively associated with 2-catechols: methylated 2-catechols (p = 0.04). Chao1 was inversely associated with E1:total estrogens (p = 0.04), and 4-pathway:2-pathway (p = 0.02) and positively associated with 2-pathway:parent estrogens (p = 0.01). Phylogenetic diversity was inversely associated with 4-pathway:total estrogens (p = 0.02), 4-pathway:parent estrogens (p = 0.03), 4-pathway:2-pathway (p = 0.01), and 4-pathway:16-pathway (p = 0.03) and positively associated with 2-pathway:parent estrogens (p = 0.01). F/B ratio was not associated with any of the estrogen measures.
Conclusion: Microbial diversity was associated with several estrogen metabolism ratios implicated in breast cancer risk. Further studies are warranted to confirm these findings in a larger and more representative sample of postmenopausal women, particularly with enrichment of minority participants.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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