Anti-capsular activity of CuO nanoparticles against Acinetobacter baumannii produce efflux pump.

Autor: Al-Kadmy IMS; Branch of Biotechnology, Department of Biology, College of Science, Mustansiriyah University, POX 10244, Baghdad, Iraq. Electronic address: israaalkadmy@gmail.com., Aziz SN; Branch of Microbiology, Department of Biology, College of Science, Mustansiriyah University, POX 10244, Baghdad, Iraq. Electronic address: sarahnaji@uomustansiriyah.edu.iq., Rheima AM; Department of Chemistry, College of Science, Mustansiriyah University, POX 10244, Baghdad, Iraq., Abid SA; Branch of Microbiology, Department of Biology, College of Science, Mustansiriyah University, POX 10244, Baghdad, Iraq. Electronic address: Suhadabas2011@gmail.com., Suhail A; Department of Physics, College of Science, Mosul University, Mosul, Iraq; Wolfson Nanomaterials & Devices Laboratory, School of Computing, Electronics and Mathematics, Faculty of Science & Engineering, Plymouth University, Devon, PL4 8AA, UK. Electronic address: ahmed.suhail@plymouth.ac.uk., Hamzah IH; Department of Biology, College of Science, Mustansiriyah University, POX 10244, Baghdad, Iraq. Electronic address: szsh@uomustansiriyah.edu.iq., Naji EN; Branch of Microbiology, Department of Biology, College of Science, Mustansiriyah University, POX 10244, Baghdad, Iraq. Electronic address: emannatiq@uomustansiriyah.edu.iq., Besinis A; Faculty of Science and Engineering, School of Engineering, University of Plymouth, Plymouth, United Kingdom. Electronic address: alexander.besinis@plymouth.ac.uk., Hetta HF; Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt. Electronic address: helalhetta@aun.edu.eg.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2023 Aug; Vol. 181, pp. 106184. Date of Electronic Publication: 2023 Jun 05.
DOI: 10.1016/j.micpath.2023.106184
Abstrakt: Copper oxide nanoparticles are modern kinds of antimicrobials, which may get a lot of interest in the clinical application. This study aimed to detect the anti-capsular activity of CuO nanoparticles against Acinetobacter baumannii produce efflux pump. Thirty-four different clinical A. baumannii isolates were collected and identified by the phenotypic and genetic methods by the recA gene as housekeeping. Antibiotic sensitivity and biofilm-forming ability, capsular formation were carried out. The effect of CuO nanoparticles on capsular isolates was detected, the synergistic effects of a combination CuO nanoparticles and gentamicin against A. baumannii were determined by micro broth checkerboard method, and the effect of CuO nanoparticles on the expression of ptk, espA and mexX genes was analyzed. Results demonstrated that CuO nanoparticles with gentamicin revealed a synergistic effect. Gene expression results show reducing the expression of these capsular genes by CuO nanoparticles is major conduct over reducing A. baumannii capsular action. Furthermore, results proved that there was a relationship between the capsule-forming ability and the absence of biofilm-forming ability. As bacterial isolates which were negative biofilm formation were positive in capsule formation and vice versa. In conclusion, CuO nanoparticles have the potential to be used as an anti-capsular agent against A. baumannii, and their combination with gentamicin can enhance their antimicrobial effect. The study also suggests that the absence of biofilm formation may be associated with the presence of capsule formation in A. baumannii. These findings provide a basis for further research on the use of CuO nanoparticles as a novel antimicrobial agent against A. baumannii and other bacterial pathogens, also to investigate the potential of CuO nanoparticles to inhibit the production of efflux pumps in A. baumannii, which are a major mechanism of antibiotic resistance.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: