Microscope-Cockpit: Python-based bespoke microscopy for bio-medical science.

Autor:	Phillips MA; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.; Diamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 0DE, UK.; Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine,, Roosevelt Drive, Oxford, OX3 7BN, UK., Susano Pinto DM; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Hall N; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Mateos-Langerak J; IGH, Univ Montpellier, Montpellier, 34396, France., Parton RM; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Titlow J; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Stoychev DV; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Parks T; Diamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 0DE, UK., Susano Pinto T; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Sedat JW; Department of Biochemistry & Biophysics, UCSF, San Francisco, CA, 94158, USA., Booth MJ; Department of Engineering Science, University of Oxford, Oxford, OX1 3PJ, UK., Davis I; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK., Dobbie IM; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.; Integrated Imaging Center, Department of Biology, Johns Hopkins University, 3400 N. Charles St., Baltimore, Maryland, 21218, USA.
Jazyk:	angličtina
Zdroj:	Wellcome open research [Wellcome Open Res] 2022 Jan 17; Vol. 6, pp. 76. Date of Electronic Publication: 2022 Jan 17 (Print Publication: 2021).
DOI:	10.12688/wellcomeopenres.16610.2
Abstrakt:	We have developed "Microscope-Cockpit" (Cockpit), a highly adaptable open source user-friendly Python-based Graphical User Interface (GUI) environment for precision control of both simple and elaborate bespoke microscope systems. The user environment allows next-generation near instantaneous navigation of the entire slide landscape for efficient selection of specimens of interest and automated acquisition without the use of eyepieces. Cockpit uses "Python-Microscope" (Microscope) for high-performance coordinated control of a wide range of hardware devices using open source software. Microscope also controls complex hardware devices such as deformable mirrors for aberration correction and spatial light modulators for structured illumination via abstracted device models. We demonstrate the advantages of the Cockpit platform using several bespoke microscopes, including a simple widefield system and a complex system with adaptive optics and structured illumination. A key strength of Cockpit is its use of Python, which means that any microscope built with Cockpit is ready for future customisation by simply adding new libraries, for example machine learning algorithms to enable automated microscopy decision making while imaging. Competing Interests: Competing interests: Martin Booth declares a significant interest in Aurox Ltd., whose microscopes were used in this work. (Copyright: © 2022 Phillips MA et al.)
Databáze:	MEDLINE
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