Cerebellar contributions to a brainwide network for flexible behavior in mice.

Autor: Verpeut JL; Neuroscience Institute, Princeton University, Washington Road, Princeton, NJ, 08544, USA. jverpeut@asu.edu., Bergeler S; Neuroscience Institute, Princeton University, Washington Road, Princeton, NJ, 08544, USA.; Department of Physics, Princeton University, Princeton, NJ, 08544, USA.; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, 08544, USA., Kislin M; Neuroscience Institute, Princeton University, Washington Road, Princeton, NJ, 08544, USA., William Townes F; Department of Statistics and Data Science, Carnegie Mellon University, Pittsburgh, PA, 15213, USA., Klibaite U; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, 01451, USA., Dhanerawala ZM; Neuroscience Institute, Princeton University, Washington Road, Princeton, NJ, 08544, USA., Hoag A; Neuroscience Institute, Princeton University, Washington Road, Princeton, NJ, 08544, USA., Janarthanan S; Neuroscience Institute, Princeton University, Washington Road, Princeton, NJ, 08544, USA., Jung C; Neuroscience Institute, Princeton University, Washington Road, Princeton, NJ, 08544, USA., Lee J; Neuroscience Institute, Princeton University, Washington Road, Princeton, NJ, 08544, USA., Pisano TJ; Neuroscience Institute, Princeton University, Washington Road, Princeton, NJ, 08544, USA., Seagraves KM; Neuroscience Institute, Princeton University, Washington Road, Princeton, NJ, 08544, USA., Shaevitz JW; Department of Physics, Princeton University, Princeton, NJ, 08544, USA.; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, 08544, USA., Wang SS; Neuroscience Institute, Princeton University, Washington Road, Princeton, NJ, 08544, USA. sswang@princeton.edu.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2023 Jun 05; Vol. 6 (1), pp. 605. Date of Electronic Publication: 2023 Jun 05.
DOI: 10.1038/s42003-023-04920-0
Abstrakt: The cerebellum regulates nonmotor behavior, but the routes of influence are not well characterized. Here we report a necessary role for the posterior cerebellum in guiding a reversal learning task through a network of diencephalic and neocortical structures, and in flexibility of free behavior. After chemogenetic inhibition of lobule VI vermis or hemispheric crus I Purkinje cells, mice could learn a water Y-maze but were impaired in ability to reverse their initial choice. To map targets of perturbation, we imaged c-Fos activation in cleared whole brains using light-sheet microscopy. Reversal learning activated diencephalic and associative neocortical regions. Distinctive subsets of structures were altered by perturbation of lobule VI (including thalamus and habenula) and crus I (including hypothalamus and prelimbic/orbital cortex), and both perturbations influenced anterior cingulate and infralimbic cortex. To identify functional networks, we used correlated variation in c-Fos activation within each group. Lobule VI inactivation weakened within-thalamus correlations, while crus I inactivation divided neocortical activity into sensorimotor and associative subnetworks. In both groups, high-throughput automated analysis of whole-body movement revealed deficiencies in across-day behavioral habituation to an open-field environment. Taken together, these experiments reveal brainwide systems for cerebellar influence that affect multiple flexible responses.
(© 2023. The Author(s).)
Databáze: MEDLINE
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