Generation and characterisation of a human induced pluripotent stem cell line, NIMHi007-A, from peripheral blood mononuclear cells derived from an adult healthy female.

Autor:	Nimonkar MM; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India., Arunachal G; Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India., Dash SS; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India., Kandy SK; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India., Raghavendra K; Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India., Chetan GK; Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India., Mehta B; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India., Markandeya YS; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India. Electronic address: yogananda@nimhans.ac.in.
Jazyk:	angličtina
Zdroj:	Stem cell research [Stem Cell Res] 2023 Aug; Vol. 70, pp. 103130. Date of Electronic Publication: 2023 May 27.
DOI:	10.1016/j.scr.2023.103130
Abstrakt:	We report the generation and characterisation of a human induced pluripotent stem cell (iPSC) line, NIMHi007-A, derived from peripheral blood mononuclear cells (PBMCs) of a healthy female adult individual. PBMCs were reprogrammed using the non-integrating Sendai virus consisting of Yamanaka reprogramming factors- SOX2, cMYC, KLF4, and OCT4. The iPSCs displayed a normal karyotype, express pluripotency markers, and could generate into three germ layers, endoderm, mesoderm, and ectoderm, in-vitro. This iPSC line, NIMHi007-A, can be used as a healthy control for various in-vitro disease models and study their underlying pathophysiological mechanisms. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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