Establishment and characterization of two novel patient-derived lines from canine high-grade glioma.

Autor: Schrock MS; Department of Radiation Oncology, Arthur G James Comprehensive Cancer Center and Richard L. Solove Research Institute, The Ohio State University, Columbus, Ohio, USA., Zalenski AA; Department of Radiation Oncology, Arthur G James Comprehensive Cancer Center and Richard L. Solove Research Institute, The Ohio State University, Columbus, Ohio, USA.; Neuroscience Graduate Program, The Ohio State University, Columbus, Ohio, USA., Tallman MM; Department of Radiation Oncology, Arthur G James Comprehensive Cancer Center and Richard L. Solove Research Institute, The Ohio State University, Columbus, Ohio, USA.; Biomedical Sciences Graduate, Program The Ohio State University Columbus, Ohio, USA., Kollin L; Department of Radiation Oncology, Arthur G James Comprehensive Cancer Center and Richard L. Solove Research Institute, The Ohio State University, Columbus, Ohio, USA., Bratasz A; Small Animal Imaging Core, The Ohio State University, Columbus, Ohio, USA., Weeks G; Small Animal Imaging Core, The Ohio State University, Columbus, Ohio, USA., Miller MA; Department of Comparative Pathobiology, Purdue University, West Lafayette, Indiana, USA., Sweeney CN; Department of Comparative Pathobiology, Purdue University, West Lafayette, Indiana, USA., Pluhar GE; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St Paul, Minnesota, USA., Olin MR; Department of Pediatrics, Division of Pediatric Hematology and Oncology, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA., Kisseberth WC; Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, Ohio, USA., Bentley RT; Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, Indiana, USA., Dickinson PJ; Department of Surgical and Radiological Sciences, UC Davis School of Veterinary Medicine, The University of California, Davis, California, USA., York D; Department of Surgical and Radiological Sciences, UC Davis School of Veterinary Medicine, The University of California, Davis, California, USA., Webb A; Department of Biomedical Informatics, The Ohio State University, Columbus, Ohio, USA., Wang X; Department of Pathobiology, Auburn University, Auburn, Alabama, USA., Moore S; Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, Ohio, USA., Venere M; Department of Radiation Oncology, Arthur G James Comprehensive Cancer Center and Richard L. Solove Research Institute, The Ohio State University, Columbus, Ohio, USA., Summers MK; Department of Radiation Oncology, Arthur G James Comprehensive Cancer Center and Richard L. Solove Research Institute, The Ohio State University, Columbus, Ohio, USA.
Jazyk: angličtina
Zdroj: Veterinary and comparative oncology [Vet Comp Oncol] 2023 Sep; Vol. 21 (3), pp. 492-502. Date of Electronic Publication: 2023 May 30.
DOI: 10.1111/vco.12912
Abstrakt: High-grade glioma is an aggressive cancer that occurs naturally in pet dogs. Canine high-grade glioma (cHGG) is treated with radiation, chemotherapy or surgery, but has no curative treatment. Within the past eight years, there have been advances in our imaging and histopathology standards as well as genetic charactereization of cHGG. However, there are only three cHGG cell lines publicly available, all of which were derived from astrocytoma and established using methods involving expansion of tumour cells in vitro on plastic dishes. In order to provide more clinically relevant cell lines for studying cHGG in vitro, the goal of this study was to establish cHGG patient-derived lines, whereby cancer cells are expanded in vivo by injecting cells into immunocompromized laboratory mice. The cells are then harvested from mice and used for in vitro studies. This method is the standard in the human field and has been shown to minimize the acquisition of genetic alterations and gene expression changes from the original tumour. Through a multi-institutional collaboration, we describe our methods for establishing two novel cHGG patient-derived lines, Boo-HA and Mo-HO, from a high-grade astrocytoma and a high-grade oligodendroglioma, respectively. We compare our novel lines to G06-A, J3T-Bg, and SDT-3G (traditional cHGG cell lines) in terms of proliferation and sensitivity to radiation. We also perform whole genome sequencing and identify an NF1 truncating mutation in Mo-HO. We report the characterization and availability of these novel patient-derived lines for use by the veterinary community.
(© 2023 The Authors. Veterinary and Comparative Oncology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje