Defining Gene Function in Spermatogonial Stem Cells Through Conditional Knockout Approaches.

Autor:	Legrand JMD; Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, Australia.; Department of Molecular and Translational Sciences, Monash University, Clayton, VIC, Australia., Hobbs RM; Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, Australia. robin.hobbs@monash.edu.; Department of Molecular and Translational Sciences, Monash University, Clayton, VIC, Australia. robin.hobbs@monash.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2656, pp. 261-307.
DOI:	10.1007/978-1-0716-3139-3_15
Abstrakt:	Mammalian male fertility is maintained throughout life by a population of self-renewing mitotic germ cells known as spermatogonial stem cells (SSCs). Much of our current understanding regarding the molecular mechanisms underlying SSC activity is derived from studies using conditional knockout mouse models. Here, we provide a guide for the selection and use of mouse strains to develop conditional knockout models for the study of SSCs, as well as their precursors and differentiation-committed progeny. We describe Cre recombinase-expressing strains, breeding strategies to generate experimental groups, and treatment regimens for inducible knockout models and provide advice for verifying and improving conditional knockout efficiency. This resource can be beneficial to those aiming to develop conditional knockout models for the study of SSC development and postnatal function. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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