Detailed mapping of Bifidobacterium strain transmission from mother to infant via a dual culture-based and metagenomic approach.

Autor: Feehily C; Teagasc Food Research Centre, Fermoy, Co, Cork, Ireland.; APC Microbiome Ireland, National University of Ireland, Cork, Ireland.; Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, United Kingdom., O'Neill IJ; APC Microbiome Ireland, National University of Ireland, Cork, Ireland.; School of Microbiology, University College Cork, Cork, Ireland., Walsh CJ; Teagasc Food Research Centre, Fermoy, Co, Cork, Ireland.; APC Microbiome Ireland, National University of Ireland, Cork, Ireland., Moore RL; UCD Perinatal Research Centre, School of Medicine, University College Dublin, National Maternity Hospital, Dublin, Ireland., Killeen SL; UCD Perinatal Research Centre, School of Medicine, University College Dublin, National Maternity Hospital, Dublin, Ireland., Geraghty AA; UCD Perinatal Research Centre, School of Medicine, University College Dublin, National Maternity Hospital, Dublin, Ireland., Lawton EM; Teagasc Food Research Centre, Fermoy, Co, Cork, Ireland.; APC Microbiome Ireland, National University of Ireland, Cork, Ireland., Byrne D; UCD Perinatal Research Centre, School of Medicine, University College Dublin, National Maternity Hospital, Dublin, Ireland., Sanchez-Gallardo R; APC Microbiome Ireland, National University of Ireland, Cork, Ireland.; School of Microbiology, University College Cork, Cork, Ireland., Nori SRC; Teagasc Food Research Centre, Fermoy, Co, Cork, Ireland.; APC Microbiome Ireland, National University of Ireland, Cork, Ireland.; SFI Centre for Research Training in Genomics Data Science, School of Mathematics, Statistics & Applied Mathematics, University of Galway, Galway, Ireland., Nielsen IB; APC Microbiome Ireland, National University of Ireland, Cork, Ireland.; School of Microbiology, University College Cork, Cork, Ireland., Wortmann E; APC Microbiome Ireland, National University of Ireland, Cork, Ireland.; School of Microbiology, University College Cork, Cork, Ireland., Matthews E; NIBRT GlycoScience Group, National Institute for Bioprocessing Research and Training, Co, Dublin, Ireland., O'Flaherty R; NIBRT GlycoScience Group, National Institute for Bioprocessing Research and Training, Co, Dublin, Ireland.; Department of Chemistry, Maynooth University, Maynooth, Co, Kildare, Ireland., Rudd PM; NIBRT GlycoScience Group, National Institute for Bioprocessing Research and Training, Co, Dublin, Ireland.; Bioprocessing Technology Institute, AStar, Singapore, Singapore., Groeger D; PrecisionBiotics Group Ltd. (Novozymes Cork), Cork Airport Business Park, Kinsale Road, Cork, Ireland., Shanahan F; APC Microbiome Ireland, National University of Ireland, Cork, Ireland., Saldova R; NIBRT GlycoScience Group, National Institute for Bioprocessing Research and Training, Co, Dublin, Ireland.; UCD School of Medicine, College of Health and Agricultural Science (CHAS), University College Dublin (UCD), Dublin, Ireland., McAuliffe FM; UCD Perinatal Research Centre, School of Medicine, University College Dublin, National Maternity Hospital, Dublin, Ireland., Van Sinderen D; APC Microbiome Ireland, National University of Ireland, Cork, Ireland. d.vansinderen@ucc.ie.; School of Microbiology, University College Cork, Cork, Ireland. d.vansinderen@ucc.ie., Cotter PD; Teagasc Food Research Centre, Fermoy, Co, Cork, Ireland.; APC Microbiome Ireland, National University of Ireland, Cork, Ireland.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 May 25; Vol. 14 (1), pp. 3015. Date of Electronic Publication: 2023 May 25.
DOI: 10.1038/s41467-023-38694-0
Abstrakt: A significant proportion of the infant gut microbiome is considered to be acquired from the mother during and after birth. Thus begins a lifelong and dynamic relationship with microbes that has an enduring impact on host health. Based on a cohort of 135 mother-infant (F = 72, M = 63) dyads (MicrobeMom: ISRCTN53023014), we investigated the phenomenon of microbial strain transfer, with a particular emphasis on the use of a combined metagenomic-culture-based approach to determine the frequency of strain transfer involving members of the genus Bifidobacterium, including species/strains present at low relative abundance. From the isolation and genome sequencing of over 449 bifidobacterial strains, we validate and augment metagenomics-based evidence to reveal strain transfer in almost 50% of dyads. Factors important in strain transfer include vaginal birth, spontaneous rupture of amniotic membranes, and avoidance of intrapartum antibiotics. Importantly, we reveal that several transfer events are uniquely detected employing either cultivation or metagenomic sequencing, highlighting the requirement for a dual approach to obtain an in-depth insight into this transfer process.
(© 2023. The Author(s).)
Databáze: MEDLINE
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