| Autor: |
Francis M; Ahmadu Bello University Zaria. markfrancis4u@gmail.com., Raji MA; Faculty of Veterinary Medicine, University of Ilorin, P.M.B 1515 Ilorin, Kwara State, Nigeria., Kwanashie CN; Faculty of Veterinary Medicine, Ahmadu Bello University P.M.B 1045 Zaria, Kaduna State, Nigeria., Adamu J; Faculty of Veterinary Medicine, Ahmadu Bello University P.M.B 1045 Zaria, Kaduna State, Nigeria., Allam L; Faculty of Veterinary Medicine, Ahmadu Bello University P.M.B 1045 Zaria, Kaduna State, Nigeria., Ameh JA; Faculty of Veterinary Medicine, University of Abuja, P.M.B 117 Gwagwalada. Abuja, Nigeria., Egwu GO; Faculty of Veterinary Medicine, University of Ilorin, P.M.B 1515 Ilorin, Kwara State, Nigeria., Zilli K; WOAH Reference Laboratory for Contagious Bovine Pleuropneumonia, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', Campo Boario, 64100 Teramo, Italy., Sacchini F; WOAH Reference Laboratory for Contagious Bovine Pleuropneumonia, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', Campo Boario, 64100 Teramo, Italy., Scacchia M; OAH Reference Laboratory for Contagious Bovine Pleuropneumonia, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', Campo Boario, 64100 Teramo, Italy. |
| Abstrakt: |
This study aimed to perform molecular typing of Mycoplasma mycoides subsp. mycoides from slaughtered cattle in Adamawa and Taraba States, north‑eastern Nigeria. A total of four hundred and eighty (480) samples of lung tissues, nasal swabs, ear swabs and pleural fluids were collected from cattle at slaughter and processed according to standard laboratory protocols. Identification and confirmation were achieved with specific PCR and PCR‑RFLP. An overall M. mycoides subsp. mycoides isolation rate of 6.87% (33/480) was obtained. In Adamawa State, 12 (10.91%) isolates of M. mycoides subsp. mycoides came from both, lung tissues and pleural fluids. While in Taraba State, 5 (7.14%) and 4 (5.71%) isolates of M. mycoides subsp. mycoides came from lung tissues and pleural fluids, respectively. The samples from nasal and ear swabs from the study states were negative for M. mycoides subsp. mycoides. Thirty‑three out of the 37 culture positive isolates were confirmed to be Mycoplasma mycoides subspecies mycoides with the production of a band equivalent to 574‑bp. Molecular typing with restriction endonuclease Vsp1 results in the two bands of 180‑bp and 380‑bp. In conclusion, the study has established an isolation rate of 6.87% for M. mycoides subsp. mycoides. Measures to strengthen movement control in order to minimise the spread of this dreaded disease of cattle were recommended. |
