Danhong injection improves neurological function in rats with ischemic stroke by enhancing neurogenesis and activating BDNF/AKT/CREB signaling pathway.

Autor: Li L; Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang province, China., Yang JH; Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang province, China., Li C; Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang province, China., Zhou HF; Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang province, China., Yu L; Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang province, China., Wu XL; Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang province, China., Lu YH; Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang province, China., He Y; Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang province, China. Electronic address: heyu0923@sina.com., Wan HT; Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang province, China. Electronic address: whtong@126.com.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2023 Jul; Vol. 163, pp. 114887. Date of Electronic Publication: 2023 May 17.
DOI: 10.1016/j.biopha.2023.114887
Abstrakt: Danhong injection (DHI) is a traditional Chinese medicine injection that promotes blood circulation and removes blood stasis and has been widely used in the treatment of stroke. Many studies have focused on the mechanism of DHI in acute ischemic stroke (IS); however, few studies have thoroughly explored its role during recovery. In this study, we aimed to determine the effect of DHI on long-term neurological function recovery after cerebral ischemia and explored the related mechanisms. Middle cerebral artery occlusion (MCAO) was used to establish an IS model in rats. The efficacy of DHI was assessed using neurological severity scores, behaviors, cerebral infarction volume and histopathology. Immunofluorescence staining was performed to assess hippocampal neurogenesis. An in vitro oxygen-glucose deprivation/reoxygenation (OGD/R) cell model was constructed and western-blot analyses were performed to verify the underlying mechanisms. Our results showed that DHI treatment greatly reduced the infarct volume, promoted neurological recovery and reversed brain pathological changes. Furthermore, DHI promoted neurogenesis by increasing the migration and proliferation of neural stem cells, and enhancing synaptic plasticity. Moreover, we found that the pro-neurogenic effects of DHI were related to an increase in brain-derived neurotrophic factor (BDNF) expression and the activation of AKT/CREB, which were attenuated by ANA-12 and LY294002, the inhibitors of the BDNF receptor and PI3K. These results suggest that DHI improves neurological function by enhancing neurogenesis and activating the BDNF/AKT/CREB signaling pathways.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier Masson SAS.)
Databáze: MEDLINE
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