Passive endocytosis in model protocells.

Autor:	Zhang SJ; Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, United States.; Department of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114, United States., Lowe LA; School of Chemistry, UNSW Sydney, Bedegal Country, New South Wales 2052, Australia.; Australian Centre for Astrobiology, UNSW Sydney, Bedegal Country, New South Wales 2052, Australia.; ARC Centre of Excellence in Synthetic Biology, UNSW Sydney, Bedegal Country, New South Wales 2052, Australia., Anees P; Neuroscience Institute, University of Chicago, Chicago, IL 60637, United States., Krishnan Y; Neuroscience Institute, University of Chicago, Chicago, IL 60637, United States.; Institute of Biophysical Dynamics, University of Chicago, Chicago, IL 60637, United States.; Department of Chemistry, University of Chicago, Chicago, IL 60637, United States., Fai TG; Department of Mathematics, Brandeis University, Waltham, Massachusetts 02453, United States., Szostak JW; Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, United States.; Department of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114, United States.; Department of Chemistry, University of Chicago, Chicago, IL 60637, United States.; Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, United States., Wang A; School of Chemistry, UNSW Sydney, Bedegal Country, New South Wales 2052, Australia.; Australian Centre for Astrobiology, UNSW Sydney, Bedegal Country, New South Wales 2052, Australia.; ARC Centre of Excellence in Synthetic Biology, UNSW Sydney, Bedegal Country, New South Wales 2052, Australia.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 May 04. Date of Electronic Publication: 2023 May 04.
DOI:	10.1101/2023.01.07.522792
Abstrakt:	Semipermeable membranes are a key feature of all living organisms. While specialized membrane transporters in cells can import otherwise impermeable nutrients, the earliest cells would have lacked a mechanism to import nutrients rapidly under nutrient-rich circumstances. Using both experiments and simulations, we find that a process akin to passive endocytosis can be recreated in model primitive cells. Molecules that are too impermeable to be absorbed can be taken up in a matter of seconds in an endocytic vesicle. The internalized cargo can then be slowly released over hours, into the main lumen or putative cytoplasm. This work demonstrates a way by which primitive life could have broken the symmetry of passive permeation prior to the evolution of protein transporters.
Databáze:	MEDLINE
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