Host-Microbiome Associations in Saliva Predict COVID-19 Severity.

Autor:	Alqedari H; Department of Oral Health Policy and Epidemiology, Harvard School of Dental Medicine, Boston, MA, 02115, USA; Dasman Diabetes Institute, Kuwait.; Dasman Diabetes Institute, 1180, Dasman, Kuwait., Altabtbaei K; School of Dentistry, Faculty of Medicine and Dentistry. University of Alberta. Edmonton AB, T6G 2L7, Canada., Espinoza JL; Department of Genomic Medicine and Infectious Diseases, J. Craig Venter Institute, La Jolla, CA 92037, USA., Bin-Hasan S; Department of Pediatrics, Farwaniyah Hospital, Ministry of Health, Kuwait., Alghounaim M; Department of Pediatrics, Amiri Hospital, Ministry of Health, Kuwait., Alawady A; Department of Pediatrics, Farwaniyah Hospital, Ministry of Health, Kuwait., Altabtabae A; Department of Pediatrics, Farwaniyah Hospital, Ministry of Health, Kuwait., AlJamaan S; Department of Pediatrics, Farwaniyah Hospital, Ministry of Health, Kuwait., Devarajan S; Dasman Diabetes Institute, 1180, Dasman, Kuwait., AlShammari T; Dasman Diabetes Institute, 1180, Dasman, Kuwait., Eid MB; Department of Pediatrics, Farwaniyah Hospital, Ministry of Health, Kuwait., Matsuoka M; Department of Genomic Medicine and Infectious Diseases, J. Craig Venter Institute, La Jolla, CA 92037, USA., Jang H; Department of Genomic Medicine and Infectious Diseases, J. Craig Venter Institute, La Jolla, CA 92037, USA., Dupont CL; Department of Genomic Medicine and Infectious Diseases, J. Craig Venter Institute, La Jolla, CA 92037, USA., Freire M; Department of Genomic Medicine and Infectious Diseases, J. Craig Venter Institute, La Jolla, CA 92037, USA.; Division of Infectious Diseases and Global Public Health Department of Medicine, University of California San Diego, La Jolla, CA, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 May 03. Date of Electronic Publication: 2023 May 03.
DOI:	10.1101/2023.05.02.539155
Abstrakt:	Established evidence indicates that oral microbiota plays a crucial role in modulating host immune responses to viral infection. Following Severe Acute Respiratory Syndrome Coronavirus 2 - SARS-CoV-2 - there are coordinated microbiome and inflammatory responses within the mucosal and systemic compartments that are unknown. The specific roles that the oral microbiota and inflammatory cytokines play in the pathogenesis of COVID-19 are yet to be explored. We evaluated the relationships between the salivary microbiome and host parameters in different groups of COVID-19 severity based on their Oxygen requirement. Saliva and blood samples (n = 80) were collected from COVID-19 and from non-infected individuals. We characterized the oral microbiomes using 16S ribosomal RNA gene sequencing and evaluated saliva and serum cytokines using Luminex multiplex analysis. Alpha diversity of the salivary microbial community was negatively associated with COVID-19 severity. Integrated cytokine evaluations of saliva and serum showed that the oral host response was distinct from the systemic response. The hierarchical classification of COVID-19 status and respiratory severity using multiple modalities separately (i.e., microbiome, salivary cytokines, and systemic cytokines) and simultaneously (i.e., multi-modal perturbation analyses) revealed that the microbiome perturbation analysis was the most informative for predicting COVID-19 status and severity, followed by the multi-modal. Our findings suggest that oral microbiome and salivary cytokines may be predictive of COVID-19 status and severity, whereas atypical local mucosal immune suppression and systemic hyperinflammation provide new cues to understand the pathogenesis in immunologically naïve populations. Competing Interests: Competing Interest Statement: All authors report there are no competing interests to disclose.
Databáze:	MEDLINE
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