Genetic manipulation of candidate phyla radiation bacteria provides functional insights into microbial dark matter.

Autor: Wang Y; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., Gallagher LA; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., Andrade PA; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., Liu A; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., Humphreys IR; Department of Biochemistry, University of Washington, Seattle, WA 98109, USA.; Institute for Protein Design, Seattle, WA 98109, USA., Turkarslan S; Institute for Systems Biology, Seattle, WA 98109, USA., Cutler KJ; Department of Physics, University of Washington, Seattle, WA 98195, USA., Arrieta-Ortiz ML; Institute for Systems Biology, Seattle, WA 98109, USA., Li Y; Department of Microbiology, University of Washington, Seattle, WA 98109, USA.; Institute for Systems Biology, Seattle, WA 98109, USA., Radey MC; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., McLean JS; Department of Microbiology, University of Washington, Seattle, WA 98109, USA.; Department of Periodontics, University of Washington, Seattle, WA 98195, USA., Cong Q; Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX, USA.; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA.; Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, USA., Baker D; Department of Biochemistry, University of Washington, Seattle, WA 98109, USA.; Institute for Protein Design, Seattle, WA 98109, USA.; Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA., Baliga NS; Institute for Systems Biology, Seattle, WA 98109, USA., Peterson SB; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., Mougous JD; Department of Microbiology, University of Washington, Seattle, WA 98109, USA.; Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.; Microbial Interactions and Microbiome Center, University of Washington, Seattle, WA 98109, USA.
Jazyk: angličtina
Zdroj: BioRxiv : the preprint server for biology [bioRxiv] 2023 May 11. Date of Electronic Publication: 2023 May 11.
DOI: 10.1101/2023.05.02.539146
Abstrakt: The study of bacteria has yielded fundamental insights into cellular biology and physiology, biotechnological advances and many therapeutics. Yet due to a lack of suitable tools, the significant portion of bacterial diversity held within the candidate phyla radiation (CPR) remains inaccessible to such pursuits. Here we show that CPR bacteria belonging to the phylum Saccharibacteria exhibit natural competence. We exploit this property to develop methods for their genetic manipulation, including the insertion of heterologous sequences and the construction of targeted gene deletions. Imaging of fluorescent protein-labeled Saccharibacteria provides high spatiotemporal resolution of phenomena accompanying epibiotic growth and a transposon insertion sequencing genome-wide screen reveals the contribution of enigmatic Saccharibacterial genes to growth on their Actinobacteria hosts. Finally, we leverage metagenomic data to provide cutting-edge protein structure-based bioinformatic resources that support the strain Southlakia epibionticum and its corresponding host, Actinomyces israelii , as a model system for unlocking the molecular underpinnings of the epibiotic lifestyle.
Databáze: MEDLINE