In vitro biocompatibility of polylactide and polybutylene succinate blends for urethral tissue engineering.

Autor: Sartoneva R; Faculty of Medicine and Health Technology (MET), Tampere University, Tampere, Finland.; Department of Obstetrics and Gynaecology, The Hospital District of South Ostrobothnia, Seinäjoki, Finland.; Tays Research Services, Wellbeing Services County of Pirkanmaa, Tampere University Hospital, Tampere, Finland., Lyyra I; Faculty of Medicine and Health Technology (MET), Tampere University, Tampere, Finland., Juusela M; Faculty of Medicine and Health Technology (MET), Tampere University, Tampere, Finland., Sharma V; Faculty of Medicine and Health Technology (MET), Tampere University, Tampere, Finland., Huhtala H; Faculty of Social Sciences, Tampere University, Tampere, Finland., Massera J; Faculty of Medicine and Health Technology (MET), Tampere University, Tampere, Finland., Kellomäki M; Faculty of Medicine and Health Technology (MET), Tampere University, Tampere, Finland., Miettinen S; Faculty of Medicine and Health Technology (MET), Tampere University, Tampere, Finland.; Tays Research Services, Wellbeing Services County of Pirkanmaa, Tampere University Hospital, Tampere, Finland.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2023 Oct; Vol. 111 (10), pp. 1728-1740. Date of Electronic Publication: 2023 May 18.
DOI: 10.1002/jbm.b.35268
Abstrakt: Surgical treatment of urothelial defects with autologous genital or extragenital tissue grafts is susceptible to complications. Tissue engineering utilizing novel biomaterials and cells such as human urothelial cells (hUC) for epithelial regeneration and adipose stromal cells (hASC) for smooth muscle restoration might offer new treatment options for urothelial defects. Previously, polylactide (PLA) has been studied for urethral tissue engineering, however, as such, it is too stiff and rigid for the application. Blending it with ductile polybutylene succinate (PBSu) could provide suitable mechanical properties for the application. Our aim was to study the morphology, viability and proliferation of hUC and hASC when cultured on 100/0 PLA/PBSu, 75/25 PLA/PBSu blend, 50/50 PLA/PBSu blend, and 0/100 PLA/PBSu discs. The results showed that the hUCs were viable and proliferated on all the studied materials. The hUCs stained pancytokeratin at 7 and 14 days, suggesting maintenance of the urothelial phenotype. The hASCs retained their viability and morphology and proliferated on all the other discs, except on PLA. On the PLA, the hASCs formed large aggregates with each other rather than attached to the material. The early smooth muscle cell markers SM22α and α-SMA were stained in hASC at 7 and 14 day time points on all PBSu-containing materials, indicating that hASCs maintain their smooth muscle differentiation potential also on PBSu. As a conclusion, PBSu is a highly potential biomaterial for urothelial tissue engineering since it supports growth and phenotypic maintenance of hUC and smooth muscle differentiation of hASC.
(© 2023 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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