Can SARS-CoV-2 screening in oral biopsies aid epidemiological surveillance?

Autor: Martins-Chaves RR; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.; Graduate Program in Medical Sciences, Faculdade Ciências Médicas de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Diniz MG; Department of Pathology, Biological Science Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Miguita L; Department of Pathology, Biological Science Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Felix FA; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Rocha FF; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Fonseca PLC; Department of Genetics, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Geddes VEV; Department of Genetics, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Menezes D; Department of Genetics, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Moreira RG; Department of Genetics, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.; Multiuser Laboratories Center, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Liborio-Kimura T; Department of Pathology and Legal Medicine, School of Medicine, Federal University of Amazonas, Manaus, Amazonas, Brazil., Câmara J; Department of Pathology and Legal Medicine, School of Medicine, Federal University of Amazonas, Manaus, Amazonas, Brazil., da Costa Mendes TC; Department of Pathology and Legal Medicine, School of Medicine, Federal University of Amazonas, Manaus, Amazonas, Brazil., Pontes HAR; Department of Oral Pathology, João de Barros Barreto University Hospital, Universidade Federal do Pará, Belém, Brazil., Pontes FSC; Department of Oral Pathology, João de Barros Barreto University Hospital, Universidade Federal do Pará, Belém, Brazil., de Freitas Gonçalves TO; Department of Oral Diagnosis and Pathology, School of Dentistry, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Fonsêca TC; Department of Oral Diagnosis and Pathology, School of Dentistry, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Abrahão AC; Department of Oral Diagnosis and Pathology, School of Dentistry, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Romañach MJ; Department of Oral Diagnosis and Pathology, School of Dentistry, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Alves APNN; Department of Dental Clinic, Faculty of Pharmacy, Dentistry, and Nursing, Universidade Federal do Ceará, Ceará, Brazil., Pereira KMA; Department of Morphology, Faculty of Medicine, Universidade Federal do Ceará, Ceará, Brazil., da Cruz Perez DE; Department of Clinical and Preventive Dentistry, Universidade Federal de Pernambuco, Recife, Brazil., de Amorim Carvalho EJ; Department of Clinical and Preventive Dentistry, Universidade Federal de Pernambuco, Recife, Brazil., Dos Santos JN; Laboratory of Oral and Maxillofacial Pathology, Dentistry School, Universidade Federal da Bahia, Salvador, Brazil., de Aquino Xavier FC; Laboratory of Oral and Maxillofacial Pathology, Dentistry School, Universidade Federal da Bahia, Salvador, Brazil., Giffoni FC; Graduate Program in Medical Sciences, Faculdade Ciências Médicas de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., de Souza AH; Graduate Program in Medical Sciences, Faculdade Ciências Médicas de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Gomes CC; Department of Pathology, Biological Science Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., de Sousa SF; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Fonseca FP; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., de Souza RP; Department of Genetics, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Aguiar RS; Department of Genetics, Biological Sciences Institute, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil., Gomez RS; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.; Graduate Program in Medical Sciences, Faculdade Ciências Médicas de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Jazyk: angličtina
Zdroj: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology [J Oral Pathol Med] 2023 Aug; Vol. 52 (7), pp. 673-679. Date of Electronic Publication: 2023 May 16.
DOI: 10.1111/jop.13438
Abstrakt: Background: Three years after the first confirmed COVID-19 case in Brazil, the outcomes of Federal government omissions in managing the crisis and anti-science stance heading into the pandemic have become even more evident. With over 36 million confirmed cases and nearly 700 000 deaths up to January 2023, the country is one of the hardest-hit places in the world. The lack of mass-testing programs was a critical broken pillar responsible for the quick and uncontrolled SARS-CoV-2 spread throughout the Brazilian population. Faced with this situation, we aimed to perform the routine SARS-CoV-2 screening through RT-qPCR of oral biopsies samples to aid in the asymptomatic epidemiological surveillance during the principal outbreak periods.
Methods: We analyzed 649 formalin-fixed paraffin-embedded oral tissue samples from five important oral and maxillofacial pathology laboratories from the north, northeast, and southeast geographic regions of Brazil. We also sequenced the whole viral genome of positive cases to investigate SARS-CoV-2 variants.
Results: The virus was detected in 9/649 analyzed samples, of which three harbored the Variant of Concern Alpha (B.1.1.7).
Conclusion: Although our approach did not value aiding asymptomatic epidemiological surveillance, we could successfully identify a using FFPE tissue samples. Therefore, we suggest using FFPE tissue samples from patients who have confirmed diagnosis of SARS-CoV-2 infection for phylogenetic reconstruction and contraindicate the routine laboratory screening of these samples as a tool for asymptomatic epidemiological surveillance.
(© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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