A systematic review about affinity tags for one-step purification and immobilization of recombinant proteins: integrated bioprocesses aiming both economic and environmental sustainability.

Autor: Leonhardt F; Food Biotechnology Laboratory, Graduate Program in Biotechnology, University of Vale do Taquari, Univates, Av. Avelino Tallini, 171, Lajeado, RS ZC 95914-014 Brazil., Gennari A; Food Biotechnology Laboratory, Graduate Program in Biotechnology, University of Vale do Taquari, Univates, Av. Avelino Tallini, 171, Lajeado, RS ZC 95914-014 Brazil., Paludo GB; Food Biotechnology Laboratory, Graduate Program in Biotechnology, University of Vale do Taquari, Univates, Av. Avelino Tallini, 171, Lajeado, RS ZC 95914-014 Brazil., Schmitz C; Food Biotechnology Laboratory, Graduate Program in Biotechnology, University of Vale do Taquari, Univates, Av. Avelino Tallini, 171, Lajeado, RS ZC 95914-014 Brazil., da Silveira FX; Federal Institute of Education, Science, and Technology of Rio Grande do Sul, IFRS, Porto Alegre Campus, Porto Alegre, RS Brazil., Moura DCDA; Federal Institute of Education, Science, and Technology of Rio Grande do Sul, IFRS, Porto Alegre Campus, Porto Alegre, RS Brazil., Renard G; Quatro G Pesquisa & Desenvolvimento Ltda, Porto Alegre, RS Brazil., Volpato G; Federal Institute of Education, Science, and Technology of Rio Grande do Sul, IFRS, Porto Alegre Campus, Porto Alegre, RS Brazil., Volken de Souza CF; Food Biotechnology Laboratory, Graduate Program in Biotechnology, University of Vale do Taquari, Univates, Av. Avelino Tallini, 171, Lajeado, RS ZC 95914-014 Brazil.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2023 Jun; Vol. 13 (6), pp. 186. Date of Electronic Publication: 2023 May 13.
DOI: 10.1007/s13205-023-03616-w
Abstrakt: The present study reviewed and discussed the promising affinity tags for one-step purification and immobilization of recombinant proteins. The approach used to structure this systematic review was The Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) methodology. The Scopus and Web of Science databases were used to perform the bibliographic survey by which 267 articles were selected. After the inclusion/exclusion criteria and the screening process, from 25 chosen documents, we identified 7 types of tags used in the last 10 years, carbohydrate-binding module tag (CBM), polyhistidine (His-tag), elastin-like polypeptides (ELPs), silaffin-3-derived pentalysine cluster (Sil3k tag), N-acetylmuramidase (AcmA tag), modified haloalkane dehalogenase (HaloTag ® ), and aldehyde from a lipase polypeptide (Aldehyde tag). The most used bacterial host for expressing the targeted protein was Escherichia coli and the most used expression vector was pET-28a. The results demonstrated two main immobilization and purification methods: the use of supports and the use of self-aggregating tags without the need of support, depending on the tag used. Besides, the chosen terminal for cloning the tag proved to be very important once it could alter enzyme activity. In conclusion, the best tag for protein one-step purification and immobilization was CBM tag, due to the eco-friendly supports that can be provided from industry wastes, the fast immobilization with high specificity, and the reduced cost of the process.
Competing Interests: Conflict of interestThe authors declare they have no conflicts of interest.
(© King Abdulaziz City for Science and Technology 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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