Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows.

Autor: Matosinho CGR; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Fonseca PAS; Centre for Genetic Improvement of Livestock, Department of Animal Biosciences, University of Guelph, Guelph, Ontario, Canada., Peixoto MGCD; Embrapa Dairy Cattle, Juiz de Fora, Minas Gerais, Brazil., Rosse IC; Departamento de Farmácia, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil., Lopes FCF; Embrapa Dairy Cattle, Juiz de Fora, Minas Gerais, Brazil., Zózimo T; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Filho AEV; Agência Paulista de Tecnologia dos Agronegócios e diretor do Laboratório de Biotecnologia do Instituto de Zootecnia do Governo do Estado de São Paulo, São Paulo, SP, Brazil., Bruneli FÂT; Embrapa Dairy Cattle, Juiz de Fora, Minas Gerais, Brazil., Carvalho MRS; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Gama MAS; Embrapa Dairy Cattle, Juiz de Fora, Minas Gerais, Brazil.
Jazyk: angličtina
Zdroj: Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie [J Anim Breed Genet] 2023 Sep; Vol. 140 (5), pp. 532-548. Date of Electronic Publication: 2023 Apr 26.
DOI: 10.1111/jbg.12777
Abstrakt: Individual variation in milk fatty acid (FA) composition has been partially attributed to stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in taurine breeds, but much less is known for Zebu breeds. This study investigated the phenotypic variation in milk FA composition, and the influence of SCD1 variants on this trait and on milk fat desaturase indices (DI) in Gir cows. The functional impact of SCD1 variants was predicted using bioinformatics tools. Milk and blood samples were collected from 312 cows distributed in 10 herds from five states of Brazil. SCD1 variants were identified through target sequencing, and milk FA composition was determined by gas chromatography. Phenotypic variation in milk FA composition fell within the range reported for taurine breeds, with SCD 18 index showing the lowest variation among the DI. Fourteen SCD1 variants were identified, six of which not previously described. Regarding the A293V polymorphism, all cows were homozygous for the C allele (coding for alanine), whereas all genotypes were detected for the second SNP affecting the 293 codon (G > A), with compelling evidence for functional effects. Significant associations (based on raw p-values) were found between this SNP and C12:0, cis-9, trans-11 CLA and short-chain FA, and between another SNP (rs523411937) and C15:0 and odd-chain linear FA. A new SNP on Chr26:21277069 was associated with trans-11 C18:1, cis-9, trans-11 CLA, C18:3 n-3 and n-3 FA. These findings indicate that SCD1 polymorphisms also contributes to the phenotypic variation in milk FA composition of Gir cows, with potential use in their breeding programmes.
(© 2023 The Authors. Journal of Animal Breeding and Genetics published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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