Role of Graphene Oxide in Bacterial Cellulose-Gelatin Hydrogels for Wound Dressing Applications.

Autor:	Khan MUA; Biomedical Research Center, Qatar University, Doha 2713, Qatar.; Department of Mechanical and Industrial Engineering, Qatar University, Doha 2713, Qatar., Stojanović GM; Faculty of Technical Sciences, University of Novi Sad, T. Dositeja Obradovi'ca 6, 21000 Novi Sad, Serbia., Hassan R; Orthodontic Unit, School of Dental Science, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, Malaysia., Anand TJS; Sustainable and Responsive Manufacturing Group, Faculty of Mechanical and Manufacturing Engineering Technology, Universiti Teknikal Malaysia Melaka, Hang Tuah Jaya, Melaka 76100, Malacca, Malaysia., Al-Ejji M; Center for Advanced Materials, Qatar University, Doha 2713, Qatar., Hasan A; Biomedical Research Center, Qatar University, Doha 2713, Qatar.; Department of Mechanical and Industrial Engineering, Qatar University, Doha 2713, Qatar.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2023 Mar 27; Vol. 8 (18), pp. 15909-15919. Date of Electronic Publication: 2023 Mar 27 (Print Publication: 2023).
DOI:	10.1021/acsomega.2c07279
Abstrakt:	Biopolymer-based hydrogels have several advantages, including robust mechanical tunability, high biocompatibility, and excellent optical properties. These hydrogels can be ideal wound dressing materials and advantageous to repair and regenerate skin wounds. In this work, we prepared composite hydrogels by blending gelatin and graphene oxide- functionalized bacterial cellulose (GO- f -BC) with tetraethyl orthosilicate (TEOS). The hydrogels were characterized using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), atomic force microscope (AFM), and water contact angle analyses to explore functional groups and their interactions, surface morphology, and wetting behavior, respectively. The swelling, biodegradation, and water retention were tested to respond to the biofluid. Maximum swelling was exhibited by GBG-1 (0.01 mg GO amount) in all media (aqueous = 1902.83%, PBS = 1546.63%, and electrolyte = 1367.32%). All hydrogels were hemocompatible, as their hemolysis was less than 0.5%, and blood coagulation time decreased as the hydrogel concentration and GO amount increased under in vitro standard conditions. These hydrogels exhibited unusual antimicrobial activities against Gram-positive and Gram-negative bacterial strains. The cell viability and proliferation were increased with an increased GO amount, and maximum values were found for GBG-4 (0.04 mg GO amount) against fibroblast (3T3) cell lines. The mature and well-adhered cell morphology of 3T3 cells was found for all hydrogel samples. Based on all findings, these hydrogels would be a potential wound dressing skin material for wound healing applications. Competing Interests: The authors declare no competing financial interest. (© 2023 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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