Detection of Mannheimia haemolytica -Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle.

Autor: Poonsuk K; Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68503, USA., Kordik C; Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68503, USA., Hille M; Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68503, USA., Cheng TY; Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA., Crosby WB; Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762, USA., Woolums AR; Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762, USA., Clawson ML; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), United States Meat Animal Research Center, Clay Center, NE 68933, USA., Chitko-McKown C; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), United States Meat Animal Research Center, Clay Center, NE 68933, USA., Brodersen B; Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68503, USA., Loy JD; Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68503, USA.
Jazyk: angličtina
Zdroj: Animals : an open access journal from MDPI [Animals (Basel)] 2023 May 03; Vol. 13 (9). Date of Electronic Publication: 2023 May 03.
DOI: 10.3390/ani13091531
Abstrakt: Mannheimia haemolytica is one of the major causes of bovine respiratory disease in cattle. The organism is the primary bacterium isolated from calves and young cattle affected with enzootic pneumonia. Novel indirect ELISAs were developed and evaluated to enable quantification of antibody responses to whole cell antigens using M. haemolytica A1 strain P1148. In this study, the ELISAs were initially developed using sera from both M. haemolytica -culture-free and clinically infected cattle, then the final prototypes were tested in the validation phase using a larger set of known-status M. haemolytica sera ( n = 145) collected from feedlot cattle. The test showed good inter-assay and intra-assay repeatability. Diagnostic sensitivity and specificity were estimated at 91% and 87% for IgG at a cutoff of S/P ≥ 0.8. IgM diagnostic sensitivity and specificity were 91% and 81% at a cutoff of sample to positive (S/P) ratio ≥ 0.8. IgA diagnostic sensitivity was 89% whereas specificity was 78% at a cutoff of S/P ≥ 0.2. ELISA results of all isotypes were related to the diagnosis of respiratory disease and isolation of M. haemolytica ( p -value < 0.05). These data suggest that M. haemolytica ELISAs can be adapted to the detection and quantification of antibody in serum specimens and support the use of these tests for the disease surveillance and disease prevention research in feedlot cattle.
Databáze: MEDLINE
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