LncRNA HCG18 promotes inflammation and apoptosis in intervertebral disc degeneration via the miR-495-3p/FSTL1 axis.

Autor: Luo Y; The Affiliated Changsha Central Hospital, Department of Spine Surgery, Hengyang Medical School, University of South China, Changsha, No. 161, The Shaoshan South Road, Changsha, 410007, Hunan, People's Republic of China. luoyily1220@163.com., He Y; The Affiliated Changsha Central Hospital, Department of Spine Surgery, Hengyang Medical School, University of South China, Changsha, No. 161, The Shaoshan South Road, Changsha, 410007, Hunan, People's Republic of China., Wang Y; The Affiliated Changsha Central Hospital, Department of Spine Surgery, Hengyang Medical School, University of South China, Changsha, No. 161, The Shaoshan South Road, Changsha, 410007, Hunan, People's Republic of China., Xu Y; The Affiliated Changsha Central Hospital, Department of Spine Surgery, Hengyang Medical School, University of South China, Changsha, No. 161, The Shaoshan South Road, Changsha, 410007, Hunan, People's Republic of China., Yang L; The Affiliated Changsha Central Hospital, Department of Spine Surgery, Hengyang Medical School, University of South China, Changsha, No. 161, The Shaoshan South Road, Changsha, 410007, Hunan, People's Republic of China.
Jazyk: angličtina
Zdroj: Molecular and cellular biochemistry [Mol Cell Biochem] 2024 Jan; Vol. 479 (1), pp. 171-181. Date of Electronic Publication: 2023 May 03.
DOI: 10.1007/s11010-023-04716-0
Abstrakt: Intervertebral disc degeneration (IDD) causes pain in the back and neck. This study investigated the role of long non-coding RNA HLA complex group 18 (HCG18) in a cell model of IDD. An IDD model was established by stimulating nucleus pulposus (NP) cells with interleukin (IL)-1β. MTT assay was performed to evaluate NP cell viability. The apoptosis was detected by flow cytometry. The expressions of HCG18, microRNA (miR)-495-3p, and follistatin-like protein-1 (FSTL1) were measured by RT-qPCR. The interactions of miR-495-3p with HCG18 and FSTL1 were analyzed by luciferase reporter assay. IL-1β stimulation upregulated HCG18 and FSTL1, but downregulated miR-495-3p in NP cells. Silencing of HCG18 or FSTL1, as well as miR-495-3p overexpression in NP cells alleviated IL-1β-induced apoptosis and inflammation of NP cells. Both HCG18 and FSTL1 had binding sites for miR-495-3p. Overexpression of FSTL1 abolished the effects of HCG18 silencing on IL-1β-induced apoptosis and inflammation. The HCG18/miR-495-3p/FSTL1 axis is essential for IDD development. Therapeutic strategies targeting this axis may be used for IDD treatment.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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