Evaluation of the yield, chemical composition and biological properties of essential oil from bioreactor-grown cultures of Salvia apiana microshoots.

Autor: Krol A; Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, al. gen. J. Hallera 107, 80-416, Gdansk, Poland., Kokotkiewicz A; Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, al. gen. J. Hallera 107, 80-416, Gdansk, Poland., Gorniak M; Department of Evolutionary Genetics and Biosystematics, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland., Naczk AM; Department of Evolutionary Genetics and Biosystematics, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland., Zabiegala B; Department of Analytical Chemistry, Faculty of Chemistry, Gdansk University of Technology, ul. Narutowicza 11/12, 80-233, Gdansk, Poland., Gebalski J; Department of Pharmaceutical Botany and Pharmacognosy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Marie Skłodowska-Curie 9, 85-094, Bydgoszcz, Poland., Graczyk F; Department of Pharmaceutical Botany and Pharmacognosy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Marie Skłodowska-Curie 9, 85-094, Bydgoszcz, Poland., Zaluski D; Department of Pharmaceutical Botany and Pharmacognosy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Marie Skłodowska-Curie 9, 85-094, Bydgoszcz, Poland., Bucinski A; Department of Biopharmacy, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. dr A. Jurasza 2, 85-089, Bydgoszcz, Poland., Luczkiewicz M; Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, al. gen. J. Hallera 107, 80-416, Gdansk, Poland. mlucz@gumed.edu.pl.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 May 02; Vol. 13 (1), pp. 7141. Date of Electronic Publication: 2023 May 02.
DOI: 10.1038/s41598-023-33950-1
Abstrakt: Microshoot cultures of the North American endemic Salvia apiana were established for the first time and evaluated for essential oil production. Stationary cultures, grown on Schenk-Hildebrandt (SH) medium, supplemented with 0.22 mg/L thidiazuron (TDZ), 2.0 mg/L 6-benzylaminopurine and 3.0% (w/v) sucrose, accumulated 1.27% (v/m dry weight) essential oil, consisting mostly of 1,8-cineole, β-pinene, α-pinene, β-myrcene and camphor. The microshoots were adapted to agitated culture, showing biomass yields up to ca. 19 g/L. Scale-up studies demonstrated that S. spiana microshoots grow well in temporary immersion systems (TIS). In the RITA bioreactor, up to 19.27 g/L dry biomass was obtained, containing 1.1% oil with up to ca. 42% cineole content. The other systems employed, i.e. Plantform (TIS) and a custom made spray bioreactor (SGB), yielded ca. 18 and 19 g/L dry weight, respectively. The essential oil content of Plantform and SGB-grown microshoots was comparable to RITA bioreactor, however, the content of cineole was substantially higher (ca. 55%). Oil samples isolated from in vitro material proved to be active in acetylcholinesterase (up to 60.0% inhibition recorded for Plantform-grown microshoots), as well as hyaluronidase and tyrosinase-inhibitory assays (up to 45.8 and 64.5% inhibition observed in the case of the SGB culture).
(© 2023. The Author(s).)
Databáze: MEDLINE
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