The neighbouring genes AvrLm10A and AvrLm10B are part of a large multigene family of cooperating effector genes conserved in Dothideomycetes and Sordariomycetes.

Autor: Talbi N; BIOGER, INRAE, Université Paris-Saclay, Palaiseau, France., Fokkens L; Molecular Plant Pathology, University of Amsterdam, Amsterdam, Netherlands., Audran C; UMR LIPME, Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France., Petit-Houdenot Y; BIOGER, INRAE, Université Paris-Saclay, Palaiseau, France., Pouzet C; FRAIB-TRI Imaging Platform Facilities, FR AIB, Université de Toulouse, CNRS, Castanet-Tolosan, France., Blaise F; BIOGER, INRAE, Université Paris-Saclay, Palaiseau, France., Gay EJ; BIOGER, INRAE, Université Paris-Saclay, Palaiseau, France., Rouxel T; BIOGER, INRAE, Université Paris-Saclay, Palaiseau, France., Balesdent MH; BIOGER, INRAE, Université Paris-Saclay, Palaiseau, France., Rep M; Molecular Plant Pathology, University of Amsterdam, Amsterdam, Netherlands., Fudal I; BIOGER, INRAE, Université Paris-Saclay, Palaiseau, France.
Jazyk: angličtina
Zdroj: Molecular plant pathology [Mol Plant Pathol] 2023 Aug; Vol. 24 (8), pp. 914-931. Date of Electronic Publication: 2023 May 01.
DOI: 10.1111/mpp.13338
Abstrakt: Fungal effectors (small-secreted proteins) have long been considered as species or even subpopulation-specific. The increasing availability of high-quality fungal genomes and annotations has allowed the identification of trans-species or trans-genera families of effectors. Two avirulence effectors, AvrLm10A and AvrLm10B, of Leptosphaeria maculans, the fungus causing stem canker of oilseed rape, are members of such a large family of effectors. AvrLm10A and AvrLm10B are neighbouring genes, organized in divergent transcriptional orientation. Sequence searches within the L. maculans genome showed that AvrLm10A/AvrLm10B belong to a multigene family comprising five pairs of genes with a similar tail-to-tail organization. The two genes, in a pair, always had the same expression pattern and two expression profiles were distinguished, associated with the biotrophic colonization of cotyledons and/or petioles and stems. Of the two protein pairs further investigated, AvrLm10A_like1/AvrLm10B_like1 and AvrLm10A_like2/AvrLm10B_like2, the second one had the ability to physically interact, similarly to what was previously described for the AvrLm10A/AvrLm10B pair, and cross-interactions were also detected for two pairs. AvrLm10A homologues were identified in more than 30 Dothideomycete and Sordariomycete plant-pathogenic fungi. One of them, SIX5, is an effector from Fusarium oxysporum f. sp. lycopersici physically interacting with the avirulence effector Avr2. We found that AvrLm10A/SIX5 homologues were associated with at least eight distinct putative effector families, suggesting that AvrLm10A/SIX5 is able to cooperate with different effectors. These results point to a general role of the AvrLm10A/SIX5 proteins as "cooperating proteins", able to interact with diverse families of effectors whose encoding gene is co-regulated with the neighbouring AvrLm10A homologue.
(© 2023 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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