Detection of SARS-CoV-2 and Its Related Factors on the Mucosal Epithelium of the Tongue.

Autor:	Tamiya J; Department of Pathology and Histomorphology, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan., Sakaguchi W; Department of Pathology and Histomorphology, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan., Nakagawa K; Department of Forensic Medicine, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan., Yamamoto T; Department of Dentistry, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan., Saruta J; Department of Education Planning, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan., Kubota N; Department of Diagnostic Pathology, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan., Kawata A; Department of Pathology and Histomorphology, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan., Hasegawa I; Department of Forensic Medicine, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan., Hamada N; Department of Oral Microbiology, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan., Tsukinoki K; Department of Pathology and Histomorphology, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan.
Jazyk:	angličtina
Zdroj:	Acta histochemica et cytochemica [Acta Histochem Cytochem] 2023 Apr 25; Vol. 56 (2), pp. 29-37. Date of Electronic Publication: 2023 Apr 08.
DOI:	10.1267/ahc.22-00089
Abstrakt:	SARS-CoV-2 infects a variety of tissues, including the oral cavity. However, there are few reports examining the association of SARS-CoV-2 with tongue mucosal tissues with sticky tongue debris. This study investigated the presence of SARS-CoV-2 and its associated molecules by dissecting tongue tissue from autopsy specimens of 23 patients who died of COVID-19-related illness (pneumonia). Immunohistochemical staining, electron microscopy, and PCR analysis were performed on the tongue tissue specimens. The mucosal epithelium of the tongue formed a very thick keratinized with well-developed filiform papillae in all cases. ACE2 and TMPRSS2 were consistently co-expressed in all samples in the epithelium. The S-protein was strongly expressed in basal cells and the epithelial surface. S-protein-positive viral particles were detected in the tongue's stratified squamous epithelium via an immunoelectron microscope. Based on PCR amplification of the N1 and N2 regions, the SARS-CoV-2 gene was detected on the tongue epithelium, tongue submucosa, and in tongue debris. This suggests that tongue debris, including the squamous epithelial tissue, could be a source of SARS-CoV-2 in saliva. Furthermore, removing tongue debris may decrease the amount of SARS-CoV-2 in the oral cavity. Competing Interests: VIThe authors declare that there are no conflicts of interest. (2023 The Japan Society of Histochemistry and Cytochemistry.)
Databáze:	MEDLINE
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