Biogenic silver nanoparticle exhibits schistosomicidal activity in vitro and reduces the parasitic burden in experimental schistosomiasis mansoni.

Autor: Detoni MB; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil. Electronic address: marianabdetoni@gmail.com., Bortoleti BTDS; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil; Graduate Program in Biosciences and Biotechnology, Carlos Chagas Institute, Fiocruz, Curitiba, Paraná, Brazil., Tomiotto-Pellissier F; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil; Graduate Program in Biosciences and Biotechnology, Carlos Chagas Institute, Fiocruz, Curitiba, Paraná, Brazil., Concato VM; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil., Gonçalves MD; Laboratory of Biotransformation and Phytochemistry, Department of Chemistry, State University of Londrina, Londrina, Paraná, Brazil., Silva TF; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil., Ortiz LSF; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil., Gomilde AC; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil., Rodrigues ACJ; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil., de Matos RLN; Laboratory of Animal Pathology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina, Paraná, Brazil., Bracarense APFRL; Laboratory of Animal Pathology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina, Paraná, Brazil., de Matos AMRN; Laboratory of Animal Pathology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina, Paraná, Brazil., Simão ANC; Clinical Analysis and Toxicology, Department of Pathology, State University of Londrina, Londrina, Paraná, Brazil., Endo TH; Laboratory of Basic and Applied Bacteriology, Department of Microbiology, State University of Londrina, Londrina, Paraná, Brazil., Kobayashi RKT; Laboratory of Basic and Applied Bacteriology, Department of Microbiology, State University of Londrina, Londrina, Paraná, Brazil., Nakazato G; Laboratory of Basic and Applied Bacteriology, Department of Microbiology, State University of Londrina, Londrina, Paraná, Brazil., Costa IN; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil., Conchon-Costa I; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil., Oliveira FJA; Laboratory of Experimental Parasitology, Department of Pathological Sciences, State University of Londrina, Paraná, Brazil., Pavanelli WR; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil., Miranda-Sapla MM; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil; Postgraduate Program in Pharmaceutical Science, University of Vale Do Itajaí, Santa Catarina, Brazil. Electronic address: mirandasapla@univali.br.
Jazyk: angličtina
Zdroj: Microbes and infection [Microbes Infect] 2023 Sep-Oct; Vol. 25 (7), pp. 105145. Date of Electronic Publication: 2023 Apr 28.
DOI: 10.1016/j.micinf.2023.105145
Abstrakt: Schistosomiasis is a neglected tropical parasitic disease that affects millions of people, being the second most prevalent parasitic disease worldwide. The current treatment has limited effectiveness, drug-resistant strains, and is not effective in different stages of the disease. This study investigated the antischistosomal activity of biogenic silver nanoparticles (Bio-AgNp) against Schistosoma mansoni. Bio-AgNp presented direct schistosomicidal activity on newly transformed schistosomula causing plasma membrane permeabilization. In S. mansoni adult worms, reduced the viability and affected the motility, increasing oxidative stress parameters, and inducing plasma membrane permeabilization, loss of mitochondrial membrane potential, lipid bodies accumulation, and autophagic vacuoles formation. During the experimental schistosomiasis mansoni model, Bio AgNp restored body weight, reduced hepatosplenomegaly, and decrease the number of eggs and worms in feces and liver tissue. The treatment also ameliorates liver damage and reduces macrophage and neutrophil infiltrates. A reduction in count and size was evaluated in the granulomas, as well as a change to an exudative-proliferative phase, with a local increase of IFN-γ. Together our results showed that Bio-AgNp is a promising therapeutic candidate for studies of new therapeutic strategies against schistosomiasis.
Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest.
(Copyright © 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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