Molecular characterization of Cryptosporidium and Giardia in environmental samples and faecal samples from biogas users in Bac Giang, Vietnam.

Autor: Nguyen TT; Department of Bacteriology, National Institute of Hygiene and Epidemiology (NIHE), 1 Yersin, Hanoi 10000, Viet Nam., Le TT; Department of Bacteriology, National Institute of Hygiene and Epidemiology (NIHE), 1 Yersin, Hanoi 10000, Viet Nam., Le HH; Department of Bacteriology, National Institute of Hygiene and Epidemiology (NIHE), 1 Yersin, Hanoi 10000, Viet Nam., Tang TN; Department of Bacteriology, National Institute of Hygiene and Epidemiology (NIHE), 1 Yersin, Hanoi 10000, Viet Nam., Phung TTH; Department of Bacteriology, National Institute of Hygiene and Epidemiology (NIHE), 1 Yersin, Hanoi 10000, Viet Nam., Vu TMH; Department of Bacteriology, National Institute of Hygiene and Epidemiology (NIHE), 1 Yersin, Hanoi 10000, Viet Nam., Tran THA; Department of Bacteriology, National Institute of Hygiene and Epidemiology (NIHE), 1 Yersin, Hanoi 10000, Viet Nam., Nguyen DT; Department of Bacteriology, National Institute of Hygiene and Epidemiology (NIHE), 1 Yersin, Hanoi 10000, Viet Nam., Nguyen TPL; Department of Bacteriology, National Institute of Hygiene and Epidemiology (NIHE), 1 Yersin, Hanoi 10000, Viet Nam., Pham DP; Center for Public Health and Ecosystem Research (CENPHER), Hanoi University of Public Health, 1A Duc Thang, Hanoi 100000, Viet Nam., Karanis P; Medical Faculty, University of Cologne, University Hospital, Cologne 50931, Germany; Department of Basic and Clinical Sciences, Medical School, University of Nicosia, Nicosia 2408, Cyprus. Electronic address: karanis.p@unic.ac.cy.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2023 Aug 01; Vol. 884, pp. 163728. Date of Electronic Publication: 2023 Apr 26.
DOI: 10.1016/j.scitotenv.2023.163728
Abstrakt: Little is known about Cryptosporidium and Giardia in biogas waste and humans in Vietnam. There is a potential risk of infections during or after using the biogas system. The detected protozoan genotypes are zoonotic pathogens, and contamination of vegetables may relay through runoff to the surface waters and soil. The objective of this study was to understand the role of the environment in the epidemiology of human infections in Bac Giang province, Vietnam, with a focus on investigating the presence of Cryptosporidium spp. genotypes and Giardia assemblages among 239 environmental samples and 94 faecal samples of biogas users. PCR and sequencing analysis were used to identify the occurrence and genotypes of Cryptosporidium and Giardia in these samples. Results showed that 13/333 (3.9 %) and 9/333 (2.7 %) samples were positive for Cryptosporidium oocyst and Giardia cysts, respectively. Characterization revealed the presence of Cryptosporidium scrofarum, C. suis, C. meleagridis, C. bailey and Giardia intestinalis assemblage A and E. C. scrofarum and Giardia assemblage E were identified for the first time in humans in Bac Giang. The current information from the above investigations will be valuable for protozoan source tracking and control interventions against Cryptosporidium and Giardia infection associated with biogas wastes in Vietnam.
Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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